Mouse anti v5 antibody

Manufactured by Immunoway

The Mouse anti-V5 antibody is a monoclonal antibody that specifically recognizes the V5 epitope tag. It is commonly used in research applications for the detection and purification of recombinant proteins expressing the V5 tag.

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Lab products found in correlation

Rabbit anti caspase 3, by Abcam (1 mentions) Mouse anti chop antibody, by Cell Signaling Technology (1 mentions) Alexa fluor 488 goat anti guinea pig, by Thermo Fisher Scientific (1 mentions) Rabbit anti p erk, by Abcam (1 mentions) Mouse anti his, by Abcam (1 mentions) Mouse anti grp78, by R&D Systems (1 mentions) Alexa fluor 568 donkey anti rabbit, by Thermo Fisher Scientific (1 mentions) Mouse anti β actin, by Merck Group (1 mentions) Alexa fluor 488 goat anti mouse, by Thermo Fisher Scientific (1 mentions) Rabbit anti grp78, by Abcam (1 mentions) Rabbit anti phospho irf3, by Cell Signaling Technology (1 mentions) Rabbit anti irf3, by Cell Signaling Technology (1 mentions) Rabbit anti phospho tbk1, by Cell Signaling Technology (1 mentions) Rabbit anti tbk1, by Abcam (1 mentions) Mouse anti pcbp2, by Santa Cruz Biotechnology (1 mentions) Rabbit anti flag, by Merck Group (1 mentions)

2 protocols using mouse anti v5 antibody

MERS-CoV and SARS-CoV-2 Protein Detection

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MERS-CoV N was detected with the in-house guinea pig anti–MERS-CoV N serum. MERS-CoV S was detected with the in-house mouse anti–MERS-CoV S immune serum. MERS-CoV M and SARS-CoV-2 M were detected with in-house mouse anti–MERS-CoV M and mouse anti–SARS-CoV-2 M immune serum, respectively. SARS-CoV M was detected with a rabbit anti–SARS-CoV M antibody from Rockland. Primary antibodies including rabbit anti–caspase-3, rabbit anti-PERK, rabbit anti-GRP78, and mouse anti-His were from Abcam. The mouse anti-CHOP antibody was from Cell Signaling Technology. The mouse anti-GRP78 was from R&D Systems. The mouse anti–β-actin was from Sigma-Aldrich. The V5-tagged proteins were detected with a mouse anti-V5 antibody from Immunoway. Secondary antibodies including Alexa Fluor 488 goat anti–guinea pig, Alexa Fluor 488 goat anti-mouse, and Alexa Fluor 568 donkey anti-rabbit were from Thermo Fisher Scientific. The goat anti-mouse horseradish peroxidase (HRP), goat anti-rabbit HRP, and goat anti–guinea pig antibodies from Thermo Fisher Scientific were used for Western blots and immunohistochemistry staining.

Chu H., Shuai H., Hou Y., Zhang X., Wen L., Huang X., Hu B., Yang D., Wang Y., Yoon C., Wong B.H., Li C., Zhao X., Poon V.K., Cai J.P., Wong K.K., Yeung M.L., Zhou J., Au-Yeung R.K., Yuan S., Jin D.Y., Kok K.H., Perlman S., Chan J.F, & Yuen K.Y. (2021). Targeting highly pathogenic coronavirus-induced apoptosis reduces viral pathogenesis and disease severity. Science Advances, 7(25), eabf8577.

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Antibodies for Immune Signaling Pathway

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Rabbit anti-phospho-IRF3 (Cat# 4947, 1:1000), rabbit anti-IRF3 (Cat# 4302, 1:1000), rabbit anti-phospho-TBK1 (Cat# 5483, 1:1000) and rabbit anti-cGAS antibodies (Cat# 15102, Cat# 79978, Cat# 31659 (Mouse specific), 1:1000) were from Cell Signaling Technology. Rabbit anti-TBK1 (Cat# ab40676, 1:1000) was from Abcam. Rabbit anti-PCBP2 (Cat# 15070-1-AP, 1:1000) was from Proteintech. Mouse anti-PCBP2 (Cat# sc-101136, 1:1000) was from Santa Cruz Biotechnology. Mouse anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Cat# KM9002, 1:4000), mouse anti-α-Tubulin (Cat# KM9007, 1:8000), and mouse anti-HA (Cat# KM8004, 1:1000) antibodies were from Sungene Biotechnology. Rabbit anti-Flag (Cat# F7425, 1:1000) antibody was from Sigma. Mouse anti-Flag (Cat# M185-3, 1:1000), rabbit anti-HA (Cat# M132-3, 1:1000), rabbit anti-Myc (Cat# 562, 1:1000), mouse anti-Myc (Cat# M192-3, 1:1000) antibodies were from MBL. Mouse anti-V5 antibody (Cat# YM3005, 1:1000) was from ImmunoWay Biotechnology Company. Rabbit anti-PCBP1 (Cat# A1044, 1:1000) antibody was from ABclonal Technology. Mouse/Rabbit anti-cGAS antibody was prepared in our laboratory, the cGAS antibody was generated by immunizing mice or rabbits with purified human cGAS full-length from E. coli.

Gu H., Yang J., Zhang J., Song Y., Zhang Y., Xu P., Zhu Y., Wang L., Zhang P., Li L., Chen D, & Sun Q. (2022). PCBP2 maintains antiviral signaling homeostasis by regulating cGAS enzymatic activity via antagonizing its condensation. Nature Communications, 13, 1564.

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