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2 protocols using poly ethylene glycol divinyl sulfone pegdvs 5 kda

1

Hydrogel Scaffold Fabrication with DTPA

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3,3 Dithiopropionic acid (DTPA), anhydrous methanol, anhydrous ethanol, hydrazine hydrate (HH), hexane, concentrated sulfuric acid, ethyl ether, hydrochloric acid (HCl), sodium hydroxide (NaOH), sodium chloride (NaCl), hyaluronic acid sodium salt (HA) from Streptococcus equi, N-3-dimethylaminopropyl-N′-ethylcarbodiimide hydrochloride (EDC), 5,5'-dithiobis-2-nitrobenzoic acid (Ellman’s reagent), and laminin-111 were purchased from Sigma Aldrich (St. Louis, MO, USA). Dithiothreitol (DTT) was purchased from Gold Biotechnology (St. Louis, MO, USA). Poly(ethylene glycol) divinyl sulfone (PEGDVS) 5 kDa was purchased from JenKem Technology USA (Allen, TX, USA).
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2

Preparation of Thiolated Hyaluronic Acid

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3,3 Dithiopropionic acid (DTPA), anhydrous methanol, anhydrous ethanol, hydrazine hydrate (HH), hexane, hyaluronic acid sodium salt (HA) from Streptococcus equi, N-3-dimethylaminopropyl-N′-ethylcarbodiimide hydrochloride (EDC), 5,5'-dithiobis-2-nitrobenzoic acid (Ellman's reagent) were purchased from Sigma Aldrich (St. Louis, MO, USA). Dithiothreitol (DTT) was purchased from Gold Biotechnology (St. Louis, MO, USA). Poly(ethylene glycol) divinyl sulfone (PEGDVS) 5 kDa was purchased from JenKem Technology USA (Allen, TX, USA).
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