Sweroside (purity > 98%, Figure 2 A) was obtained from National Institutes for Food and Drug Control (Beijing, China). Fetal bovine serum (FBS) and phosphate buffer saline (PBS) were acquired from Servicebio Company (Wuhan, China). DMEM, DMSO, trypsin, the RIPA Lysis buffer and the PVDF membrane were obtained from Solarbio Company (Beijing, China). The annexin V-FITC apoptosis detection kit was obtained from Vazyme Biotech Company (Nanjing, China). Hoechst 33342, NAM, SIRT1, NF-κB, β-actin and the proliferating cell nuclear antigen (PCNA) antibodies were from Beyotime Institute of Biotechnology (Shanghai, China). The TNF-α, IL-1β, IL-6, IL-10, MnSOD, FOXO1, COX-2 and i-NOS antibodies were obtained from Proteintech Company (Wuhan, China). The ROS assay kit, CCK-8 assay kit, NO assay kit, cell cycle and apoptosis analysis kits were from Beyotime Institute of Biotechnology (Shanghai, China). The PGE2 Elisa Kit was purchased from Hui Jia Company (Xiamen, China). The other chemicals were of analytical grade and were commercially available.
Phosphate buffer saline (pbs)
Manufactured by Wuhan Servicebio Technology
Sourced in China
Phosphate buffer saline is a commonly used buffer solution in various laboratory applications. It is composed of sodium phosphate and sodium chloride, and its primary function is to maintain a stable and physiologically relevant pH environment for biological samples and experiments.
Automatically generated - may contain errors
Lab products found in correlation
Ros assay kit, by Beyotime (2 mentions)
Interleukin 6 (il 6), by Proteintech (1 mentions)
Il 1β, by Proteintech (1 mentions)
Cell cycle and apoptosis analysis kit, by Beyotime (1 mentions)
Pvdf membrane, by Solarbio (1 mentions)
Il 10, by Proteintech (1 mentions)
Fetal bovine serum (fbs), by Wuhan Servicebio Technology (1 mentions)
Sirt1, by Beyotime (1 mentions)
Mnsod, by Proteintech (1 mentions)
Foxo1, by Proteintech (1 mentions)
Nf κb, by Beyotime (1 mentions)
Hoechst 33342, by Beyotime (1 mentions)
β actin, by Beyotime (1 mentions)
Ripa lysis buffer, by Solarbio (1 mentions)
Tnf α, by Proteintech (1 mentions)
Trypsin, by Solarbio (1 mentions)
Dimethyl sulfoxide (dmso), by Solarbio (1 mentions)
Annexin 5 fitc apoptosis detection kit, by Vazyme (1 mentions)
No assay kit, by Beyotime (1 mentions)
Cck 8 assay kit, by Beyotime (1 mentions)
8 protocols using phosphate buffer saline (pbs)
1
Sweroside Modulates Inflammatory Response
2
Synthesis and Characterization of BLM Nanoparticles
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BLM was purchased from Acmec biochemical (China). Bovine serum albumin (BSA) and EGCG were purchased from Macklin (China). FeCl3·6H2O was purchased from Aladdin (China). Phosphate buffer saline (PBS) was purchased from Servicebio (China). Penicillin–streptomycin solution (100 ×), fetal bovine serum (FBS), RPMI medium 1640, and trypsin were purchased from Gibco (USA). 4′,6-diamidino-2-phenylindole (DAPI), fluorescent dye 1,1′-dioctadecyl-3,3,3′-dio tetramethylindotricarbocyanine iodide (DiR) and ROS assay kit were obtained from Beyotime (China). Ethanol absolute, ammonia solution, ethylenediamine tetraacetic acid (EDTA), urea, glucose and all other chemical reagents were purchased from Sinopharm Chemical Reagent Co., Ltd (China).
3
Tetrodotoxin HPLC Purification Protocol
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Tetrodotoxin (HPLC 99%) was supplied by Zhongyang Biotechnology Co., Ltd. (Shanghai, China). Formic acid, acetic acid and acetonitrile from Sinopharm Group Chemical Reagent Co., Ltd. (Shanghai, China) were analytical-grade. Phosphate buffer saline (PBS) and paraformaldehyde fixative were purchased from Servicebio Technology Co., Ltd. (Wuhan, China). Glucose serum (5%) was from Chenxin Pharmaceutical Co., Ltd. (Jining, China). Primary secondary amine (PSA) and capped octadecyl silica gel (C18) powder were obtained from Agela Technologies (Torrance, USA). Distilled water was purified using a water purification system (Elix, Merck Millipore, Molsheim, France). Food for mice in conformity with Chinese national standard (GB 14924.3-2010) was purchased from the Laboratory Animal Center at the Naval Medical University (Shanghai, China).
4
Anlotinib Cell Culture Assay
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F12k culture medium, phosphate buffer saline (PBS), Penicillin–streptomycin double antibody and normal saline were procured from Servicebio (Wuhan, China). Fetal bovine serum and 0.25% trypsin–EDTA were purchased from Gibico (Waltham, MA, USA). Dimethyl sulfoxide was obtained from Macklin (Shanghai, China). HPLC-grade methanol, acetonitrile, formic acid and MS-grade ammonium acetate were procured from Fisher Scientific. Dichloromethane was obtained from Merck (Darmstadt, Germany). Standards of analytes and internal standards were derived from Sigma Aldrich (St. Louis, MO, USA). Anlotinib was obtained from Chia Tai Tianqing Pharmaceutical Group Co.,Ltd. The drug was dissolved in Dimethyl sulfoxide (DMSO) for preparation of stock solution, where the final concentration of DMSO in the drug working solution in the cells was < 0.01%. DMSO of 0.01% was used as a vehicle control in all cell culture assays.
5
Cytotoxicity Evaluation of Reagents
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All reagents and solvents, including aloin, arginine, and alginate were purchased from Aladdin (Shanghai, China) and used without further purification unless otherwise specified. Phosphate buffer saline (PBS) was purchased from Servicebio (Wuhan, China). A Live/Dead viability/cytotoxicity assay kit and Cell Counting Kit-8 (CCK-8) solution were purchased from Proteintech (Wuhan, China). Fetal bovine serum (FBS) and Dulbeccos modified Eagle's medium (DMEM) were provided by Gibco (USA).
6
Corilagin and Aspirin Evaluation for Cellular and Animal Studies
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Corilagin standard substance (SC9500, purity ≥ 98%) for cells and aspirin for animals (A8830, purity ≥ 98%) were purchased from Solarbio (Beijing, China). Corilagin for animal experimentation (N0272, purity 90% ~ 99%) was obtained from Hengcheng Zhiyuan Biotechnology (Sichuan, China). Aspirin for cells (B21505, purity ≥ 98%) was purchased from Yuanye Biotechnology (Shanghai, China). Oxidized low-density lipoprotein (Ox-LDL) was purchased from Yiyuan Biotechnology (YB-002, Guangzhou, China). Lipopolysaccharides (LPS, L8880) was purchased from Solarbio. Fetal bovine serum (FBS, 10099–141) and Roswell Park Memorial Institute (RPMI)-1640 were obtained from Gibco (New York, USA). Phosphate buffer saline (PBS) was obtained from Servicebio (G4202, Wuhan, China) and 4% paraformaldehyde (PFA) was purchased from Biosharp (BL520, Guangzhou, China).
7
Lipid Profiling of Biological Samples
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The reference standards of LPC(13:0) (internal standard, IS), LPC(14:0), LPC(16:0), LPC(17:0), LPC(18:1) and LPC(20:0) were purchased from Avanti Polar Lipids Inc. (Alabaster, AL, USA). Mass spectrometry grade-methanol and acetonitrile were obtained from Merck Inc. (Darmstadt, HE, Germany). Analytical reagent-chloroform and HPLC grade-formic acid were purchased from Xilong Technology Co., Ltd. (Shantou, China). Phosphate buffer saline (PBS) was supplied by Servicebio Technology Co., Ltd. (Wuhan, China). Bovine serum albumin (BSA) was provided by Energy Chemical Technology Co., Ltd. (Shanghai, China). CH and APAP were purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Isoflurane was supplied by RWD Life Science CO., Ltd. (Shenzhen, China)
8
Detailed Hepatotoxicity Evaluation Protocol
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UA (purity ≥ 93%), α-naphthalene-isothiocyanate (ANIT, purity 98%), and chloral hydrate (purity > 99%) were purchased from Shanghai Macklin Biochemical Technology Co., Ltd (Shanghai, China). Alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphokinase (ALP), and γglutamyl transferase (γ-GT) assay kits were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Total bilirubin (T-BIL), direct bilirubin (D-BIL), and total bile acid (TBA) assay kits were provided by Changchun Huili Biotechnology Co., Ltd (Changchun, China). Hematoxylin staining solution, tissue xative (4% paraformaldehyde), and phosphate buffer saline (PBS) were from Servicebio Biotechnology Co., Ltd (Wuhan, China). Antibodies for GAPDH, UGT2B7, and MRP2 were purchased from Proteintech Group (USA). Antibodies for BSEP and Nrf2 were provided by Abcam (UK). Antibodies for horseradish peroxidase-labeled goat anti-rabbit IgG and goat anti-mouse IgG were from Proteintech Group (Wuhan, China)
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