Phenylephrine and ach

Glucose was measured using a FreeStyle Lite® (Abbott) glucometer with accompanying strips, and triglyceride assay reagents were procured from Sigma. Insulin (Humulin R, Lilly) was purchased at a local pharmacy. Insulin ELISA kits were from ALPCO Diagnostics. S-Nitro-N-acetyl-DL-penicillamine (SNAP) and cyclic guanosine monophosphate (cGMP) were procured from Cayman Chemical Company. Sodium chloride, potassium chloride, calcium chloride, magnesium chloride, sodium bicarbonate, potassium phosphate, D-glucose, collagenase, ethylenediaminetetraacetic acid (EDTA), ethylene glycol tetraacetic acid (EGTA), sodium pyrophosphate, sodium orthovanadate, sodium fluoride, okadaic acid, 1% protease inhibitor cocktail, dithiothreitol, magnesium chloride, K-lactobionate, taurine, potassium phosphate, HEPES, digitonin, pyruvate, malic acid, glutamic acid, adenosine diphosphate, succinic acid, oligomycin, carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone, phenylephrine and ACh, trypsin inhibitor, and cytochrome c were procured from Sigma-Aldrich (MO, USA). Mammalian Protein Extraction Reagent (M-PER) was from Thermo Scientific, and Immobilon-P PVDF membrane was from Millipore. Sepiapterin was procured from Sigma-Aldrich.