We used 123 non-duplicate clinical isolates of E. coli collected in the University Hospital of Besançon (France) between 2011 and 2016 and stored at the Centre de Ressources Biologiques Filière Microbiologique, Besançon (CRB-FMB, Biobanque BB-0033-00090). Table 1 details the susceptibility of E. coli isolates to AMX and CTX. We seeded blood-containing vials (BD BACTEC plus Aerobic/F medium, Becton Dickinson, Sparks, USA) with E. coli isolates for a final load of 106 CFU. Vials were incubated at 35°C in an automated blood culture system (BACTEC FX Blood Culture System, Becton Dickinson) until its detection.
Bactec plus aerobic f medium
Manufactured by BD
Sourced in United States, United Kingdom, Germany
The BD BACTEC plus Aerobic/F medium is a laboratory culture medium used for the detection of aerobic microorganisms in blood samples. It is designed to be used in conjunction with the BD BACTEC blood culture system.
Automatically generated - may contain errors
6 protocols using bactec plus aerobic f medium
1
Detecting E. coli Growth in Blood Cultures
2
Prospective Study of Blood Culture Sampling
3
Automated Blood Culture Procedure
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The study was performed from September to October 2021 at Peking Union Medical College Hospital. Blood cultures (BACTEC™ PLUS-Aerobic/F Medium, BACTEC™-Lytic/10 Anaerobic/F Medium, and BACTEC™-Myco/F Lytic Medium; BD Diagnostics, Oxford Science Park, Oxford, United Kingdom) were sent to the microbiology laboratory. Distribution of patients was across different departments including emergency, intensive care unit, respiratory disease, cardiology, immunology, infectious diseases, neurology, and oncology. Aerobic/F and Anaerobic/F bottles were incubated in the BACTEC™ FX system for 5 days, and Myco/F bottles were incubated for 14 days for yeasts, such as Candida. The acceptable time period from bottle collection to placement onto the automated blood culture instruments should be within 2 h, and all of the bottles detected in the present study fulfilled this criteria.
4
Febrile Patient Blood Culture Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Incubated blood culture material had been collected from a total of 2324 BD (Becton Dickinson, Heidelberg, Germany) blood culture bottles (BACTEC Plus Aerobic/F Medium for adults and BD BACTEC Peds Plus Medium for children) that had been inoculated with blood from febrile Ghanaian patients in the course of a two-year study period from 2007 to 2008 [24 (link),25 (link),26 (link),27 (link),28 (link)]. In short, the bottles were filled according to the manufacturer’s instructions and incubated at 36 °C until a positive result was indicated or otherwise for a total of 5 days as detailed elsewhere [24 (link)]. Culture-based growth of bacteria succeeded from 12.7% of the samples, while the inclusion of panbacterial 16S rRNA DNA gene-specific PCR increased the proportion of positive samples to 36.7% [25 (link)]. Residual samples of the collected blood culture material had been stored at −80 °C and shipped on dry ice to Germany for molecular assessments.
5
Microbial Culturing of Tissue and Bone Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Tissue specimens were homogenized using a stomacher (Seward Inc., Port St. Lucie, FL). Before March 2016, the homogenates were inoculated onto sheep-blood and chocolate agar, incubated aerobically at 35°C in 5% CO2 for 5 days, and onto CDC anaerobic blood agar and into a prereduced thioglycollate broth, incubated anaerobically for 14 days. From April 2016 onwards, homogenates were inoculated into blood culture bottles (BD BACTEC Plus Aerobic/F medium and BD BACTEC Lytic/10 Anaerobic/F medium) and incubated on the BACTEC 9240/FX instruments (BD Diagnostic Systems) for 14 days. Bone specimens were processed through a grinder with brain-heart infusion broth. Grounded bone specimens were inoculated into agar plates.
6
Blood Culture Collection Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Whole blood samples (8–10 ml) were collected from the patients and inoculated into each vial of the blood culture set, which includes an aerobic bottle (BD BACTEC Plus Aerobic/F Medium) and an anaerobic bottle (BD BACTEC Lytic/10 Anaerobic/F), according to the hospital policy for taking blood samples for blood culture. Blood culture vials were incubated into the BACTEC blood culture analyser (BD BACTEC FX) within 2 h of taking blood samples.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!