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Photometrics cascade 512b ccd camera

Manufactured by Teledyne

The Photometrics Cascade 512B is a CCD camera designed for scientific and industrial applications. It features a 512 x 512 pixel array and a 16-bit analog-to-digital converter. The camera can operate at frame rates up to 30 frames per second and has a high quantum efficiency.

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2 protocols using photometrics cascade 512b ccd camera

1

Evaluating Mitochondrial Function in Fibroblasts

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Fibroblasts from the affected sibling harbouring the SLC25A1 variant c.740G > A; p.R247Q and control fibroblasts were grown in DMEM (high glucose). Membrane potential was measured by fluorescence microscopy using the tetramethylrhodamine methyl ester (TMRM) probe [22 (link)], while mitochondrial morphology was investigated using MitoTracker® Red CMXRos. MitoTracker® Red CMXRos and tetramethylrhodamine methyl ester (TMRM) were purchased from Life Technologies.
Fluorescent images of treated cells were acquired and fluorescence intensities were analyzed, as previously described [23 (link)], using a Zeiss Axiovert 200 microscope equipped with a Photometrics Cascade 512B CCD camera (Roper Scientific) and the MetaFluor software (Universal Imaging).
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2

Evaluating Mitochondrial Function in Fibroblasts

Check if the same lab product or an alternative is used in the 5 most similar protocols
Fibroblasts from the affected sibling harbouring the SLC25A1 variant c.740G > A; p.R247Q and control fibroblasts were grown in DMEM (high glucose). Membrane potential was measured by fluorescence microscopy using the tetramethylrhodamine methyl ester (TMRM) probe [22 (link)], while mitochondrial morphology was investigated using MitoTracker® Red CMXRos. MitoTracker® Red CMXRos and tetramethylrhodamine methyl ester (TMRM) were purchased from Life Technologies.
Fluorescent images of treated cells were acquired and fluorescence intensities were analyzed, as previously described [23 (link)], using a Zeiss Axiovert 200 microscope equipped with a Photometrics Cascade 512B CCD camera (Roper Scientific) and the MetaFluor software (Universal Imaging).
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