Fluorescent images of treated cells were acquired and fluorescence intensities were analyzed, as previously described [23 (link)], using a Zeiss Axiovert 200 microscope equipped with a Photometrics Cascade 512B CCD camera (Roper Scientific) and the MetaFluor software (Universal Imaging).
Photometrics cascade 512b ccd camera
The Photometrics Cascade 512B is a CCD camera designed for scientific and industrial applications. It features a 512 x 512 pixel array and a 16-bit analog-to-digital converter. The camera can operate at frame rates up to 30 frames per second and has a high quantum efficiency.
2 protocols using photometrics cascade 512b ccd camera
Evaluating Mitochondrial Function in Fibroblasts
Fluorescent images of treated cells were acquired and fluorescence intensities were analyzed, as previously described [23 (link)], using a Zeiss Axiovert 200 microscope equipped with a Photometrics Cascade 512B CCD camera (Roper Scientific) and the MetaFluor software (Universal Imaging).
Evaluating Mitochondrial Function in Fibroblasts
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