Blood samples and bone marrow aspirate for immunophenotype analysis was obtained prior to plerixafor administration on day -5 and after its administration on day -4 (prior to thiotepa administration). The cell content was phenotyped by flow cytometry using BD FACSCanto™ II flow cytometer, BD FACSDiva 6.0 software, and a red cell lysis/multi-color antibody protocol. The following monoclonal antibodies against cell surface or intracellular markers were used: Anti-CD45 APC-H7 (Clone 2D1), Anti-CD33 PE-Cy7 (Clone P67.6), Anti-sCD3 V450 (Clone UCHT1), Anti-CD7 FITC (Clone M-T701), Anti-CD5 PE-Cy7 (Clone L17F12), Anti-CD19 APC (Clone SJ25C1), Anti-CD33 APC (Clone P67.6), Anti-HLADR APC-H7 (Clone L243), Anti-CD184 (CXCR4) PE (Clone ID9), Anti-IgG 2a PE (Clone X-39; all 6from BD Biosciences, San Jose, CA); Anti-CD34 PerCP (Clone 581), Anti-cCD3 PerCP (Clone SK7; all from Biolegend, San Diego, CA); Anti-CD38 FITC (Clone T16; Beckman Coulter, Pasadena, CA) and Anti-CD133 APC (Clone AC133, Miltenyi Biotec, Cambridge, MA). Patient-specific combinations of 6 or 8 antibodies and Boolean gating scheme were used to identify the blasts for each patient and determine their CXCR4 expression. Matched isotype control was used to determine the upper limit of fluorescent background.
Anti cd34 percp
Manufactured by BioLegend
Sourced in Macao
Anti-CD34 PerCP is a fluorescently-labeled antibody that binds to the CD34 antigen. CD34 is a cell surface glycoprotein expressed on hematopoietic stem and progenitor cells. This antibody can be used to identify and quantify CD34-positive cells in flow cytometry applications.
Automatically generated - may contain errors
Lab products found in correlation
Facsdiva 6, by BD (1 mentions)
Anti cd19 apc, by BD (1 mentions)
Anti igg2a pe, by BD (1 mentions)
Anti cd133 apc, by Miltenyi Biotec (1 mentions)
Anti cd45 apc h7, by BD (1 mentions)
Anti cd33 pe cy7, by BD (1 mentions)
Anti cd5 pe cy7, by BD (1 mentions)
Anti cd33 apc, by BD (1 mentions)
Anti hladr apc h7, by BD (1 mentions)
Anti cd38 fitc, by Beckman Coulter (1 mentions)
Anti cd184 cxcr4 pe, by BD (1 mentions)
Facscanto 2 flow cytometer, by BD (1 mentions)
Fitc conjugated, by Abcam (1 mentions)
Accuri flow cytometer, by BD (1 mentions)
Anti cd38 apc, by BioLegend (1 mentions)
Anti c1q, by Abcam (1 mentions)
Daratumumab, by Johnson & Johnson (1 mentions)
2 protocols using anti cd34 percp
1
Immunophenotypic Analysis of Leukemia Cells
2
Characterization of Daratumumab Binding
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Daratumumab was from Janssen Pharmaceuticals (Titusville, NJ), isotype control (human IgG1 kappa) from Sigma-Aldrich (St Louis MO), and anti-CD38-APC, anti-CD34-PerCP, anti-CD59-FITC (H19 clone) and isotype controls from BioLegend (San Diego, CA). Second antibody for Daratumumab binding was mouse anti-human IgG Fc APC-conjugated (HP6017, BioLegend). The anti-C1q was a rabbit polyclonal FITC-conjugated (Abcam, Cambridge, MA) used with an appropriate isotype control. BRIC 229, a CD59 neutralizing antibody, was obtained from the International Blood Group Reference Laboratory of the Bristol Institute for Transfusion Sciences (NHS Blood and Transplant, Bristol, UK), and the anti-CD46 monoclonal GB24 was kindly provided by Dr. J. Aktinson, Washington University, St. Louis, MO, USA. Antibodies were titrated for optimal use and analyses performed on a BD Accuri flow cytometer (BD Biosciences, San Jose, CA).
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