Led light engine

Manufactured by Lumencor

The LED light engine is a compact and efficient illumination source that utilizes light-emitting diodes (LEDs) to generate high-intensity, customizable light output. It is designed to provide a stable, long-lasting, and energy-efficient solution for a variety of laboratory and scientific applications.

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Lab products found in correlation

Gm 8000, by Tokai Hit (2 mentions) Dmi6000b, by Leica camera (2 mentions) Ti e inverted microscope, by Oxford Instruments (1 mentions) Deltavision elite standard filter set, by Olympus (1 mentions) Scmos camera, by Olympus (1 mentions) Ixon3 emccd camera, by Oxford Instruments (1 mentions) Ix71 inverted microscope, by Olympus (1 mentions) Dichroic mirror 495, by Leica camera (1 mentions)

3 protocols using led light engine

Fluorescence Microscopy Imaging Protocol

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For microscopy, cells were grown to log phase in SD supplemented with appropriate amino acids mixes, and then briefly pelleted and resuspended to concentrate prior to imaging. Images for Fig 2 were captured using a DeltaVision^TM Elite system (GE Healthcare Life Sciences), equipped with an Olympus IX-71 inverted microscope, a sCMOS CAMERA, A 100x/1.4 Oil Super-Plan APO objective, and a DeltaVision Elite Standard Filter Set with the FITC filter (excitation:475/28, emission:525/48) for GFP and the TRITC filter (excitation: 542/27, emission: 594/45) for mCh. Image acquisition and deconvolution were performed with the program softWoRx. All other fluorescence imaging, was performed on a Nikon Ti-E inverted microscope with a 1.4 numerical aperture, 100× oil-immersion objective and an Andor iXon3 EMCCD camera. A Lumencor LED light engine was used for fluorophore excitation. Image acquisition was performed the MetaMorph acquisition software. For each field, 10-slice Z-stacks (0.25 μm apart) were captured. For image cropping and other figure preparation steps, the ImageJ version 1.50i (National Institutes of Health) software was used.

Martínez-Márquez J.Y, & Duncan M.C. (2018). Investigation of Ldb19/Art1 localization and function at the late Golgi. PLoS ONE, 13(11), e0206944.

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Live-Cell Imaging of Light-Induced Interactions

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Prior to imaging, the culture medium was changed to an imaging medium derived from ref. ^{23 (link)} containing increased glucose to prevent starvation-induced filopodia:^{45 (link)} 20 mM HEPES at pH 7.5, 150 mM NaCl, 5 mM KCl, 1 mM CaCl2, 1 mM MgCl2 and 4.5 g/L glucose supplemented with 10% fetal bovine serum. Live cell imaging was performed on an epifluorescence microscope (Leica DMI6000B) equipped with an on-stage CO2 incubation chamber (Tokai Hit GM-8000) and a motorized stage (Prior). An adaptive focus control was used to actively keep the image in focus during the period of imaging. A light-emitting diode (LED) light engine (Lumencor) was used as the light source for fluorescence imaging. Pulsed blue light (200 ms pulse duration at 5.3 W/cm²) was delivered every 10 s both to image GFP-tagged constructs and to initiate iLID/SspB interactions. Pulsed green light (200 ms pulse duration) was used to image mCherry or tdTomato. The microscope was equipped with a commercial GFP filter cube (Leica; excitation filter 472/30, dichroic mirror 495, emission filter 520/35) and a commercial Texas red filter cube (Leica; excitation filter 560/40, dichroic mirror 595, emission filter 645/75). All Images were acquired with an oil-immersion 100× objective. All experiments were imaged with a sensitive CMOS camera (PCO.EDGE 5.5) (PCO).

Jones T I.V., Liu A, & Cui B. (2020). Light-inducible generation of membrane curvature in live cells with engineered BAR domain proteins. ACS synthetic biology, 9(4), 893-901.

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Visualizing Light-Induced Protein Interactions

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Prior to imaging, the culture medium was changed to an imaging medium derived from ref. 23 containing increased glucose to prevent starvation-induced filopodia: 45 20 mM HEPES at pH 7.5, 150 mM NaCl, 5 mM KCl, 1 mM CaCl2, 1 mM MgCl2 and 4.5 g/L glucose supplemented with 10% fetal bovine serum. Live cell imaging was performed on an epifluorescence microscope (Leica DMI6000B) equipped with an on-stage CO2 incubation chamber (Tokai Hit GM-8000) and a motorized stage (Prior). An adaptive focus control was used to actively keep the image in focus during the period of imaging. A light-emitting diode (LED) light engine (Lumencor) was used as the light source for fluorescence imaging. Pulsed blue light (200 ms pulse duration at 5.3 W/cm 2 ) was delivered every 10 s both to image GFP-tagged constructs and to initiate iLID/SspB interactions. Pulsed green light (200 ms pulse duration) was used to image mCherry or tdTomato. The microscope was equipped with a commercial GFP filter cube (Leica; excitation filter 472/30, dichroic mirror 495, emission filter 520/35) and a commercial Texas red filter cube (Leica; excitation filter 560/40, dichroic mirror 595, emission filter 645/75). All Images were acquired with an oil-immersion 100× objective. All experiments were imaged with a sensitive CMOS camera (PCO.EDGE 5.5) (PCO).

Jones T., Liu A., & Cui B. (2020). Light-inducible generation of membrane curvature in live cells with engineered BAR domain proteins.

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