The paraffin-embedded kidney was sectioned at 3 μm using a rotary microtome (Leica ASP 300S, Germany). The tissue sections were then prepared on the glass slides and placed on a hot plate (HI1220; Leica Microsystems). The tissue sections were then deparaffinized using xylene and rehydrated by immersing them in a series of decreasing concentrations of ethanol (100%, 90%, and 70%). The sections later were stained with H&E to observe the changes in kidney structures.
Hi1220
Manufactured by Leica Microsystems
The HI1220 is a high-performance digital camera module designed for microscopy applications. It features a 12-megapixel CMOS sensor, providing high-resolution imaging capabilities. The camera supports a wide range of interfaces, including USB 3.0 and GigE Vision, enabling seamless integration with various microscope systems. The HI1220 delivers reliable and consistent image capture, making it a suitable solution for a variety of microscopy workflows.
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Lab products found in correlation
3 protocols using hi1220
1
Histological Analysis of Kidney Tissue
2
Histological Analysis of Organ Samples
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The collected organs including, aorta, liver, kidneys, spleen, adrenals, and brain samples were sliced into small sections of about 0.5–2 cm thickness and were fixed in 10% formalin for at least 48 hours. The fixed samples were placed in plastic cassettes and dehydrated using an automated tissue processor (ASP300; Leica Microsystems, Wetzlar, Germany). The tissues were embedded in paraffin wax (EG1160; Leica Micro-systems), and the blocks were trimmed and sectioned to 4 µm using a semiautomated microtome (RM2155; Leica Micro-systems). Then, the tissue sections were mounted on glass slides using a hot plate (HI1220; Leica Microsystems).
Subsequently, the tissue sections were deparafinized by two changes of xylene for 5 minutes each and rehydrated by three changes of different graded ethanol dilution (100%, 90%, and 70%) for 5 minutes each, respectively. Afterward, the sections were stained with Harris’s hematoxylin and eosin (HE) method, as described previously.28 Finally, tissue sections were mounted with glass cover slips using mounting medium distyrene-plasticizer xylene and were examined using a light microscope image analyzer (BX51TF; Olympus Corporation, Tokyo, Japan).
Subsequently, the tissue sections were deparafinized by two changes of xylene for 5 minutes each and rehydrated by three changes of different graded ethanol dilution (100%, 90%, and 70%) for 5 minutes each, respectively. Afterward, the sections were stained with Harris’s hematoxylin and eosin (HE) method, as described previously.28 Finally, tissue sections were mounted with glass cover slips using mounting medium distyrene-plasticizer xylene and were examined using a light microscope image analyzer (BX51TF; Olympus Corporation, Tokyo, Japan).
3
Histological Analysis of Paraffin-Embedded Pancreas
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The paraffin-embedded pancreas was sectioned at 4 μm using a semiautomated microtome (RM2155; Leica Micro-systems). The tissue sections were then mounted on glass slides using a hot plate (HI1220; Leica Microsystems). Afterward, the tissue sections were deparafinized by xylene and rehydrated by different graded ethanol dilution (100%, 90%, and 70%). The sections were stained with hematoxylin and eosin (H&E). All slides were examined using light microscopy (Motic BA410, Wetzlar, Germany) equipped with a digital camera (Moticam Pro 285A, Wetzlar, Germany) under a magnification of X200.
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