Phospho nrf2

Arachidonic acid (AA), PD98059, SB203580, and SP600125, were obtained from Calbiochem (Billerica, MA, USA), while NAD(P)H quinone oxidoreductase-1 (NQO-1), lamin A/C, and caspase-3 antibodies were provided by Cell Signaling (Danvers, MA, USA). Bad antibody was obtained from BD Bioscience (Becton Dickinson Biosciences, Mountain View, CA, USA). Antibodies against Nrf2, phospho-Nrf2, Bax, Bcl-xL, and Poly (ADP-ribose) polymerase (PARP) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Hemoxygenase (HO)-1 antibody was purchased from Enzo Life Sciences (Plymouth Meeting, PA, USA) and glutamate cysteine ligase (GCL) antibody from Abcam (Cambridge, MA, USA). Rottlerin, compound C, phenylhydrazine (PHZ), 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA), dimethyl sulfoxide, and β-actin antibody were provided by Sigma (St. Louis, MO, USA). The iron-NTA complex (1:3 Fe/NTA) was prepared as described previously [24 (link)].

Antibodies against Nrf2, phospho-Nrf2, Bax, and Bcl-xL were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). NQO1, lamin A/C, and caspase-3 antibodies were obtained from Cell Signaling (Danvers, MA, USA). Glutamate cysteine ligase (GCL) antibody was purchased from Abcam (Cambridge, UK) and a Bad antibody was purchased from BD biosciences (Franklin Lakes, NJ, USA). MTT, metaphosphoric acid, DCFH-DA, t-butylhydroperoxide (t-BHP), rotenone, dimethylsulfoxide, and β-actin antibody were purchased from Sigma-Aldrich (St. Louis, MO, USA). siNrf2 was purchased from Dharmacon (Lafayette, CO, USA).