Unmineralized samples were attached to a petri dish using a drop of cyanoacrylate adhesive, and left for a minute for the adhesive to dry. Samples were then immersed in distilled water. Young’s modulus measurements were taken with a JPK Nanowizard-1 (JPK Instruments, Germany) in force spectroscopy mode, mounted on an inverted optical microscope (IX-81; Olympus, Japan). Quadratic pyramidal cantilevers (MLCT; Bruker, MA, USA) with a spring constant of 0.07 N/m and half-angle to face of 17.5° were used for indentation. The sensitivity of cantilevers was determined before measurements by measuring the slope of the force-distance curve in the atomic force microscopy (AFM) software on an empty region of a petri dish. Indentation was carried out with an approach speed of 5 µm/s and a maximum set force of 1 nN. Measurements were taken multiple times per region and in multiple regions per sample. The Young’s modulus was calculated by fitting the contact region of the approach curve with the Hertz Contact model66 (link) using the JPK software, using the above constants and calibrated cantilever sensitivity. Graphs were plotted with GraphPad Prism software, using a P-value of 0.05.
Quadratic pyramidal cantilevers mlct
Manufactured by Bruker
Sourced in United States
Quadratic pyramidal cantilevers (MLCT) are a type of atomic force microscopy (AFM) probe designed for high-resolution imaging and force measurements. The cantilevers feature a quadratic pyramidal tip geometry, providing high aspect ratio and sharp apex for enhanced resolution. This probe is suitable for a range of AFM applications, including imaging and force spectroscopy.
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Lab products found in correlation
2 protocols using quadratic pyramidal cantilevers mlct
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Nanomechanical Measurements of Unmineralized Samples
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Measuring Mechanical Properties of Biological Samples
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Samples were attached to a petri dish using a drop of cyanoacrylate adhesive, and left for a minute for the adhesive to dry. Samples were then immersed in distilled water. Young's Modulus measurements were taken with a JPK Nanowizard-1 (JPK Instruments, Germany) in force spectroscopy mode, mounted on an inverted optical microscope (IX-81; Olympus, Japan). Quadratic pyramidal cantilevers (MLCT; Bruker, MA, USA) with a spring constant of 0.07 N/m and half-angle to face of 17.5°were used for indentation. The sensitivity of cantilevers was determined before measurements by measuring the slope of the force-distance curve in the AFM software on an empty region of a petri dish. Indentation was carried out with an approach speed of 5 mm/s and a maximum set force of 1 nN. Measurements were taken multiple times per region and in multiple regions per sample. The Young's Modulus was calculated by fitting the contact region of the approach curve with the Hertz Contact model [25] using the JPK software, using the above constants and calibrated cantilever sensitivity.
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