Embryos for culture were dissected in M2 culture media (Millipore) keeping the ectoplacental cone intact, removing Reichart’s membrane, then transferred to a glass-bottomed culture dish in 50% rat serum/50% DMEM/F12 and incubated at 37°C with 5% CO2 for 12 h in 5µM Tubacin diluted in DMSO.
M2 culture media
Manufactured by Merck Group
M2 culture media is a commonly used laboratory reagent designed to support the growth and maintenance of cells in vitro. It is a balanced salt solution that provides the necessary nutrients, vitamins, and other components to sustain cell cultures. The core function of M2 culture media is to create an optimal environment for the cultivation and propagation of a variety of cell types.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using m2 culture media
1
Embryo Culture with Tubacin
2
Embryo Culture with Tubacin
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!