Fr167653

Adriamycin was obtained from Sigma Aldrich (St. Luis, MO). FR167653, p38α MAPK inhibitor, was kindly provided by Astellas Pharma Inc. (Tokyo, Japan). Primary antibodies used for immunohistochemical studies and Western blotting were mouse anti-NPR-C (1:100, sc-515449; SantaCruz, Dallas, TX), goat anti-nephrin (1:100, AF3159; R&D Systems, Minneapolis, MN), rabbit anti-monocyte chemotactic protein-1 (1:100, ab25124; Abcam, Cambridge, UK), rabbit anti-phospho-p38 MAPK (1:1000, #9211; Cell Signaling Technology, Boston, MA), mouse anti-desmin (1:150, M0760; DAKO, Tokyo, Japan), mouse anti-β actin (1:1000, A5411; Sigma-Aldrich), and rat anti-MAC-2 (1:100, CL8942F; Cedarlane, CA).

Aldosterone was obtained from Sigma Aldrich (St. Louis, MO). FR167653, p38α MAP Kinase inhibitor, was kindly provided by Astellas Pharma Inc. (Tokyo, Japan). Primary antibodies used for immunohistochemical studies and Western blotting were goat anti-nephrin (R&D Systems, Minneapolis, MN), rabbit anti-podocin (Sigma Aldrich), rabbit anti-phospho-p38 MAPK (Cell Signaling Technology, Boston, MA), rabbit total p38 MAPK (Cell Signaling Technology), rabbit anti-WT1 (Santa Cruz, Dallas, TX), and rabbit anti-p53 (Vector Laboratories, Burlingame, CA), rabbit anti-phospho-MKP-1 (Cell Signaling Technology), rabbit anti-phospho-MKK3 (Cell Signaling Technology), and mouse anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH; Santa Cruz) antibodies. pRc/RSV Flag MKK350 (link) and pRc/RSV Flag MKK3 (glu)51 (link) were gifts from Professor Roger Davis (Addgene plasmid #14671 and #14670, respectively).