Accutof jms t100lc mass spectrometer

GC-MS was performed on a QP5050A system (Shimadzu Corp., Kyoto, Japan) using an Equity-5 column (0.25 mm i.d. × 30 m, 0.25 μm film thickness; Supelco, Inc., Bellefonte, PA, USA). The HPLC consisted of a 626 pump with 996 photodiode array detector (Waters Corp., Milford, MA) equipped with a C-18 column (Inertsil ODS-3, 5 μm, 250 × 4.6 mm ID; GL Sciences, Inc., Tokyo, Japan). ¹H and ¹³C NMR spectra were recorded with tetramethylsilane as the internal standard using JNM-EX270 (270 MHz) and JNM-LA500 (500 MHz) NMR spectrometers (JEOL Ltd., Tokyo, Japan). High resolution mass spectra were obtained with a JMS-T100LC AccuTOF mass spectrometer (JEOL). The seeds used in the present study have been previously described6 (link). They were planted at a depth of 5 mm in pots (8 × 8 × 6 cm deep) containing sand and then incubated in an illuminated growth chamber (FLI-301N; Tokyo, Rikakikai, Co., Ltd., Tokyo, Japan) under a 16-h light/8-h dark cycle at 22 °C.

The melting points (uncorrected) were determined on a Fisher-Jhons apparatus (Fisher Scientific Company, Pittsburgh, PA, USA). The optical rotations were measured on a Perkin-Elmer 323 polarimeter (Perkin Elmer Inc., London, UK). The UV spectra were recorded on a Shimadzu UV 160U spectrophotometer (Shimadzu, Kyoto, Japan). VCD data were acquired on a BioTools dualPEM ChiralIR FT-VCD spectrophotometer (Jupiter, FL, USA). The IR spectra were obtained on a Bruker Tensor 27 spectrometer (Bruker, Ettlingen, Germany); 1D and 2D NMR experiments were performed on a Bruker Advance III HD spectrometer (Bruker Corporation, Billerica, MA, USA) at 700 MHz for ¹H and 175 MHz for ¹³C. Chemical shifts were referred to CDCl₃ (δ_H = 7.26, δ_C = 77.16). The HR-DART-MS data were acquired on a Jeol, AccuTOF JMS-T100LC mass spectrometer (Jeol Ltd., Tokyo, Japan); silica gel 230–400 mesh (Macherey-Nagel, Macherey Nagel, Düren, Germany), Sephadex LH-20 (Pharmacia Biotech AB, Uppsala, Sweden), and octadecyl-functionalized silica gel (Sigma-Aldrich, St. Louis, MO, USA) were used for column chromatography. The X-ray data were collected on an Agilent Xcalibur Atlas Gemini diffractometer (Agilent Technologies, Oxfordshire, UK).