Fluoview 1000 confocal laser scanning system
The FluoView 1000 is a confocal laser scanning system designed for high-resolution imaging. It utilizes laser excitation and a focused detection system to capture detailed fluorescence images of biological samples.
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8 protocols using fluoview 1000 confocal laser scanning system
Analyzing Mitophagy in C2C12 Cells
Immunofluorescence Assay for DRG2 in Tissue Sections
Histological and Immunofluorescence Analysis
For immunofluorescence (IF) staining of tissue and primary adipocytes, snap-frozen tissues were sectioned using a cryostat and then defrosted at room temperature for 1 h. The primary adipocytes and tissue sections were fixed in 4% paraformaldehyde (HT5014; Sigma-Aldrich) at room temperature for 20 min, washed thrice in PBS, incubated with 0.2% Triton X-100 for 5 min, and rinsed in PBS. The sections were blocked with 5% goat serum with 0.1% Triton X-100, whereas adipocytes were blocked with 3% bovine serum albumin (BSA) for 1 h at room temperature. Then, both were incubated with the target protein antibody (1:200) with their respective blocking solution overnight at 4 °C. The next day, the tissues and adipocytes were again washed thrice with PBS, stained with Alexa Fluor-conjugated secondary antibody in blocking solution at room temperature in the dark for 1 h, washed twice with PBS, and incubated with 10 μM DAPI in PBS at room temperature for 10 min. Images were acquired and analyzed using an Olympus FluoView 1000 confocal laser scanning system. The slides were scored in a blinded manner.
Visualizing Mitochondrial Morphology in Cells Expressing DJ-1 Variants
Confocal Microscopy Analysis of Photosynthetic Pigments
Immunofluorescence Analysis of DNA Damage
Biofilm Viability Assessment by Microscopy and Staining
Viability was assessed: (1) by inoculating biofilm fragments (about 25 mm2) into 2 ml of liquid BG-11 medium and measuring cell densities with a spectrophotometer after 3 months of growth under routine conditions and (2) by staining with 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyl tetrazolium chloride (Sigma Aldrich, Saint Louis, MO, USA) after rehydration for 30 min, 60 min, and 72 h, as previously reported (Billi, 2009 (link)).
Confocal Microscopy for Mitochondrial Analysis
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