PG was collected from Jilin Province in China and purchased from Zhejiang Yingte Pharmaceutical Co., Ltd. (Hangzhou, China). PN was collected from Yunnan Province in China and purchased from KPC Pharmaceuticals, Inc. (Kunming, China). Both root plants were identified by Xingchu Gong, an associate professor at the College of Pharmaceutical Sciences at Zhejiang University, according to the Chinese Pharmacopoeia. Reference ginsenosides Rg1, Re, Rf, Rb1, Rc, Ro, Rb2, Rb3, Rd, and notoginsenoside R1 were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Analytical grade ethanol was purchased from Shanghai Lingfeng Chemical Reagent Co., Ltd. (Shanghai, China). Chromatographic-grade methanol and acetonitrile were purchased from Merck (Darmstadt, Germany) and that of phosphoric acid was purchased from the Tedia Company (Fairfield, IA, USA). Deionized water was obtained using a Milli-Q academic water purification system (Millipore, Milford, MA, USA).
Notoginsenoside r1
Manufactured by Yuanye Bio-Technology
Sourced in China
Notoginsenoside R1 is a bioactive compound isolated from the Chinese medicinal plant Panax notoginseng. It is a saponin glycoside with potential applications in research and development. The core function of Notoginsenoside R1 is to serve as a reference standard for analysis and identification purposes.
Automatically generated - may contain errors
Lab products found in correlation
Ginsenoside rg1, by Yuanye Bio-Technology (3 mentions)
Ginsenoside rb1, by Yuanye Bio-Technology (2 mentions)
Ginsenoside rb3, by Yuanye Bio-Technology (2 mentions)
Ginsenoside rd, by Yuanye Bio-Technology (2 mentions)
20 s ginsenoside rg3, by Yuanye Bio-Technology (2 mentions)
Ginsenoside rc, by Yuanye Bio-Technology (2 mentions)
Milli q academic water purification system, by Merck Group (1 mentions)
Chromatographic grade methanol and acetonitrile, by Merck Group (1 mentions)
Acetonitrile, by Thermo Fisher Scientific (1 mentions)
Lipopolysaccaride lps, by Merck Group (1 mentions)
Griess reagent, by Promega (1 mentions)
Ginsenoside ro, by Yuanye Bio-Technology (1 mentions)
Ginsenoside rb2, by Yuanye Bio-Technology (1 mentions)
Ginsenoside rg2, by Yuanye Bio-Technology (1 mentions)
20r ginsenoside rg3, by Yuanye Bio-Technology (1 mentions)
Milli q water purification system, by Merck Group (1 mentions)
Cell counting kit 8 cck 8, by Biosharp (1 mentions)
Dextran sulfate sodium (dss), by MP Biomedicals (1 mentions)
Acetonitrile, by Tedia (1 mentions)
A549 cell, by American Type Culture Collection (1 mentions)
6 protocols using notoginsenoside r1
1
HPLC Analysis of Ginseng Constituents
2
Quantification of Ginsenoside Compounds
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Notoginsenoside R1 (R1), ginsenoside Rb1 (Rb1), ginsenoside Rb2 (Rb2), ginsenoside Rb3 (Rb3), ginsenoside Rg1 (Rg1), ginsenoside Rg2 (Rg2), ginsenoside Rf (Rf), ginsenoside Ro (Ro), ginsenoside Rd (Rd), ginsenoside Re (Re), (20S)-ginsenoside Rg3 ((20S)-Rg3), (20R)-ginsenoside Rg3 ((20R)-Rg3), ginsenoside Rk2 (Rk2) (≥98% pure) were provided by the Shanghai Yuanye Biotechnology Co., ltd. (Shanghai, China). P. ginseng, A. macrocephala, P. cocos and G. uralensis applied in the study were purchased from Fusong Shenyuan Changbaishan Ginseng Technology Co., ltd. (Fusong, China).
Acetonitrile was purchased from Fisher Scientific (Waltham, MA, USA). Water was purified through a Milli-Q water purification system (Millipore, MA, USA). TNF-α, IL-1β, IL-6 and NO ELISA kits were provided by Jiangsu Mai Sha Industry Co., ltd (Yancheng, China). Dextran sulfate sodium (DSS) was obtained from MP Biomedicals (California, USA). Cell Counting Kit-8 (CCK-8) was obtained from Biosharp Life Science (Shanghai, China). Lipopolysaccaride (LPS) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Griess reagents were purchased from Promega (Madison, USA).
Acetonitrile was purchased from Fisher Scientific (Waltham, MA, USA). Water was purified through a Milli-Q water purification system (Millipore, MA, USA). TNF-α, IL-1β, IL-6 and NO ELISA kits were provided by Jiangsu Mai Sha Industry Co., ltd (Yancheng, China). Dextran sulfate sodium (DSS) was obtained from MP Biomedicals (California, USA). Cell Counting Kit-8 (CCK-8) was obtained from Biosharp Life Science (Shanghai, China). Lipopolysaccaride (LPS) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Griess reagents were purchased from Promega (Madison, USA).
3
Comparison of American Ginseng Samples
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Four samples of P. quinquefolius were obtained from Wendeng (Weihai, China), Yanbian (China), Simcoe, ON, (Canada), and Dane County, WI (USA). The botanical origin of the materials was identified by Professor Kechun Liu, Biology Institute of Shandong Academy of Sciences (Jinan, China). Water (Watsons Ltd., Hong Kong, China) and acetonitrile (Tedia Company Inc., Fairfield, OH, USA) were of LC–MS grade. All other chemicals were of analytical grade. Ginsenoside Rb3, notoginsenoside R1, and ginsenoside Rc were purchased from Shanghai Yuanye Biotechnology Co., Ltd (Shanghai, China). The A549 cells were acquired from the American Type Culture Collection (ATCC) (Rockville, MD, USA).
4
Saponin Content Quantification in P. notoginseng
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Dry root samples of 0.3 g were extracted in 100% methanol and sonicated for 30 min. The solution volume was fixed to 25 mL. Saponin contents were determined as described by Pharmacopoeia of People’s Republic of China (2020) . Saponin contents were measured using a high-performance liquid chromatograph (Agilent 1260; Agilent Technologies, Santa Clara, CA, USA). Notoginsenoside R1, ginsenoside Rd, ginsenoside Rg1, ginsenoside Re, and ginsenoside Rb1 standards ( > 98% purity) were purchased from Yuanye Bio-technology (Shanghai, China). Kit column (250 mm × 4.6 mm, 5 μm) was used for the determination, and the mobile phase was acetonitrile (ACN)-water. Chromatographic conditions: elution with 0–5 min, 17–20% ACN; 5–20 min, 20% ACN; 20–45 min, 20–42% ACN; 45–50 min, 42–100% ACN; set with flow rate, injection volume, monitoring wavelength and column temperature of 1.0 mL·min−1, 10 µL, 203 nm and room temperature, respectively. Total saponins are the sum of Rg1, Rb1, Re, Rd and R1. The HPLC chromatograms of P. notoginseng root grown in different N environments are shown in Fig. S3 .
5
Fingerprinting Ginseng Compounds using RRPG
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RRPG was supplied by the Scientific Research Institute of Beijing Tongrentang Co., Ltd. Total 35 reference standards, including ginsenoside Re5 (R20 ), Re3 (R28 ), Re4 (R34 ), Re1 (R46 ), Re2 (R54 ), Rg1 (R73 ), Re (R74 ), Rf (R200 ), Ra2 (R248 ), Ra3 (R257 ), Rb1 (R259 ), Ra1 (R289 ), Ro (R301 ), Rb2 (R324 ), Rb3 (R335 ), Rs2 (R401 ), Rd (R406 ), Ro methyl ester (R493 ), Rg6 (R556 ), F4 (R566 ), 20-gluco-ginsenoside Rf (R40 ), notoginsenoside R1 (R50 ), R4 (R201 ), R2 (R216 ), quinquenoside R1 (R358 ), ginsenjilinol (R70 ), 20(S)-ginsenoside Rg2 (R261 ), 20(S)-ginsenoside Rh1 (R263 ), 20(R)-ginsenoside Rg2 (R281 ), ginsenoside Rc (R285 ), 20(R)-ginsenoside Rh1 (R286 ), 20(S)-ginsenoside Rg3 (R593 ), 20(R)-ginsenoside Rg3 (R599 ), 20(S)-ginsenoside-Rh2 (R619 ) and 20(R)-ginsenoside-Rh2 (R620 ), were either purchased from the National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China) as well as Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China) or gifts from the State Key Laboratory of Natural and Biomimetic Drugs, Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University. The purity of all the reference standards were >98%. LC-MS-grade acetonitrile as well as methanol was obtained from Merck (Darmstadt, Germany), LC-MS grade formic acid was purchased from Fisher-Scientific (Fair Lawn, NJ, USA), and distilled water was obtained from Watsons.
6
Protective Effects of HDAX on ER Stress
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HDAX were mainly composed of Coptidis Rhizoma, Radix Rehmanniae, Radix Puerariae, Radix Ophiopogonis, Eriobotryae Folium, and Radix Notoginseng, which were purchased from Anhui Provincial Hospital of Chinese Medicine. Notoginsenoside R1, ginsenoside Rg1, puerarin, acteoside, berberine, coptisine, and palmatine hydrochloride were purchased from Shanghai yuanye Bio-Technology Co., Ltd (Shanghai, China). 4-phenylbutyric acid (4-PBA) was purchased from Sigma-Aldrich (St. Louis, MO, USA). The primary antibody of GRP78, CHOP was purchased from A nity Bioscience (1:1300, USA); Caspase-12, Caspase-9, Caspase-3 were obtained from Bioss (1:1000, Beijing, China); Bax, Bcl-2 was provided by Bioworld (1:1000, Nanjing, China).
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