Standard IHC techniques were used to probe intestinal tissues for zonulin expression using the following antibodies: sheep anti-mouse haptoglobin primary antibody (Invitrogen PA5-33158); rabbit anti-sheep HRP conjugated secondary antibody (Abcam Ab97130). Details of the experimental methods can be found in online supplementary material . All images used in this study have been uploaded to the Mouse Model of Type 1 Diabetes Atlas (MMDA)30 and are available for viewing and discussion online (mmda.lib.miamioh.edu).
Ab97130
Manufactured by Abcam
Ab97130 is a product offered by Abcam, a leading provider of high-quality reagents and tools for researchers. The core function of this product is to serve as a tool for scientific investigation, without further interpretation or extrapolation on its intended use.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using ab97130
1
Intestinal Zonulin Expression Profiling
2
Antibody Validation via Western Blot
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Standard Western blots were used to confirm reactivity of the antibodies to haptoglobin and zonulin using the following antibodies: sheep anti-mouse haptoglobin primary (Invitrogen PA5-33158); rabbit anti-sheep horseradish peroxidase (HRP) conjugated secondary (Abcam Ab97130). Detailed methods for the Western blot experiments can be found in online supplementary material .
3
Quantification of CSF1 and Transferrin in Plasma
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Plasma was separated from EDTA whole blood by centrifugation at 2000 G for 15 min at 4°C. A 1:50 dilution was prepared using SDS-PAGE loading buffer. Proteins were separated by SDS-PAGE and transferred to nitrocellulose membranes for immunoblotting. Membranes were probed with polyclonal primary Abs: rabbit anti-CSF1 (1:500, MBS551037; MyBioSource) and 1:2000 sheep anti-Transferrin (1:2000, ab9033; Abcam) in PBS/3% milk powder/0.1% (v/v) Tween 20 at 4°C overnight. The secondary Abs used were goat anti-rabbit HRP (1:5000, no. 7074; Cell Signaling Technologies) and rabbit anti-sheep HRP (1:10,000, ab97130; Abcam). Blots were visualized using Pierce ECL Western blotting solution (Thermo Fisher Scientific).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!