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Protein g sensor chip

Manufactured by GE Healthcare
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The Protein G sensor chip is a lab equipment product designed for use in affinity-based biosensor systems. The sensor chip is coated with Protein G, a bacterial protein that binds to the Fc region of immunoglobulins. This allows the chip to be used for the detection and quantification of antibodies in samples.

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Lab products found in correlation

Biacore t200, by GE Healthcare (2 mentions) Biacore t200, by Cytiva (2 mentions) Biacore t200 evaluation software version 3, by GE Healthcare (1 mentions) Glycine hcl ph 1, by GE Healthcare (1 mentions) Hbs ep, by GE Healthcare (1 mentions) Glycine 1, by GE Healthcare (1 mentions) Glycine hcl, by GE Healthcare (1 mentions) Biacore 8k, by GE Healthcare (1 mentions) Biacore t200 evaluation software v3, by Cytiva (1 mentions)

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Series S Protein G sensor chip (5 citations) Sensor chips (2 citations)

5 protocols using protein g sensor chip

1

Binding Kinetics of Monoclonal Antibodies to HSA

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The affinity and kinetic of Mab binding to HSA was determined by BiacoreTM T200 (GE Healthcare life sciences, Illinois, USA), a Surface Plasmon Resonance instrument. HBS‐EP+ (GE Healthcare life sciences, Illinois, USA) was used as a system running buffer. Goat anti‐mouse IgG1 (Sigma‐Aldrich, Missouri, USA) was immobilized on the surface of the Protein G sensor chip (GE Healthcare life sciences, Illinois, USA). All ten clones of MAbs (ligand) were diluted to the concentration of 3 µg/ml with running buffer. A concentration of 20 nM and 320 nM of HSA (analyte) was injected with a flow rate of 30 µl/min for 30 s. The Protein G sensor chip surface was regenerated with 10 mM Glycine HCl pH 1.5 (GE Healthcare life sciences, USA). The results were analyzed with BIAcore T200 evaluation software version 3.1 (GE Healthcare life sciences, USA).
Vutthikraivit N., Kiatamornrak P., Boonkrai C., Pisitkun T., Komolpis K., Puthong S., Lumlertgul N., Peerapornratana S., Thanawattano C., Tungsanga S., Praditpornsilpa K., Tungsanga K., Eiam‐Ong S, & Srisawat N. (2021). Development and validation of point‐of‐care testing of albuminuria for early screening of chronic kidney disease. Journal of Clinical Laboratory Analysis, 35(4), e23729.
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2

SPR Analysis of IL4 Receptor Binding

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SPR measurements were performed using a Biacore ×100 SPR system (GE Healthcare). Human IL4 receptor alpha-FC chimera (Biolegend) was immobilized on a protein G sensor chip (GE Healthcare). Log2 dilution concentration series consisted of apoA1–IL4 ranging from 200 nM to 6.25 nM and of human IL4 ranging from 20 nM to 0.65 nM. All samples were prepared in HPS-EP buffer (10 mM HEPES, 150 mM NaCl, 3 mM EDTA, 0.005% (v/v) P20 pH 7.4). Association was monitored for 180 s and dissociation for 180 s with a flow rate of 30 μl min−1. Sensor chip was regenerated with glycine 1.5 (10 mM glycine-HCl pH 1.5, GE Healthcare). Kinetics was determined by fitting the interaction SPR data for 1:1 binding.
Schrijver D.P., Röring R.J., Deckers J., de Dreu A., Toner Y.C., Prevot G., Priem B., Munitz J., Nugraha E.G., van Elsas Y., Azzun A., Anbergen T., Groh L.A., Becker A.M., Pérez-Medina C., Oosterwijk R.S., Novakovic B., Moorlag S.J., Jansen A., Pickkers P., Kox M., Beldman T.J., Kluza E., van Leent M.M., Teunissen A.J., van der Meel R., Fayad Z.A., Joosten L.A., Fisher E.A., Merkx M., Netea M.G, & Mulder W.J. (2023). Resolving sepsis-induced immunoparalysis via trained immunity by targeting interleukin-4 to myeloid cells. Nature Biomedical Engineering, 7(9), 1097-1112.
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3

Quantifying Antibody Binding Affinity to N9 Neuraminidase

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Purified anti-N9 mAbs binding affinity to SH13 N9 NA was measured using a Biacore 8K instrument (GE Healthcare Life Sciences). Briefly, IgGs were captured on a Protein G sensor chip (GE Healthcare Life Sciences) with final surface densities of ~245 to 400 relative units (RU). Kinetic measurements were preformed using single-cycle kinetics experimental setup, by injecting a 0.156 μg/mL solution of IgG, and then bound with four-fold serial dilutions of N9 NA (starting from 100 nM). Dissociation data for IgGs were collected for 10 min. Binding data were globally fit to a bivalent analyte model (using Biacore 8K control software). This analysis determined the kinetic rate constants (Kon, Koff), from which the apparent KD was then calculated as Koff/Kon. Dissociation % indicates percent of IgG dissociated from antigen at time of dissociation step, according to relative unit (RU) values.
Gilchuk I.M., Bangaru S., Gilchuk P., Irving R.P., Kose N., Bombardi R.G., Thornburg N.J., Creech C.B., Edwards K.M., Li S., Turner H.L., Yu W., Zhu X., Wilson I.A., Ward A.B, & Crowe JE J.r. (2019). Influenza H7N9 Virus Neuraminidase-Specific Human Monoclonal Antibodies Inhibit Viral Egress and Protect from Lethal Influenza Infection in Mice. Cell host & microbe, 26(6), 715-728.e8.
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4

Binding Kinetics of Anti-SARS-CoV-2 Antibodies

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To determine the binding kinetics of human monoclonal antibodies disclosed herein and RBD of wild-type SARS-CoV-2 spike protein, the surface plasmon resonance (SPR) technique was performed by Biacore T200 equipped with a protein G sensor chip (GE Healthcare). For the antibody-capturing step, each purified human anti-SARS-CoV-2 monoclonal antibody (at 1 μg/ml prepared in the HBS-EP buffer) was injected into an individual flow cell in the sensor chip. Single-cycle kinetics at 25°C was determined by sequentially injecting recombinant RBD-His tag proteins at different concentrations. An HBS-EP buffer blank was also included as a negative control for baseline subtraction. The antibody binding affinity (KD) value was calculated using the Biacore T200 Evaluation Software v3.1.
Boonkrai C., Cotrone T.S., Chaisuriyong W., Tantawichien T., Thisyakorn U., Fernandez S., Hunsawong T., Reed M., Wongtangprasert T., Audomsun T., Phakham T., Attakitbancha C., Saelao P., Focht D., Kimbung R., Welin M., Malik A.A., Pisitkun T, & Srisawat N. (2023). Efficacy of the combination of monoclonal antibodies against the SARS-CoV-2 Beta and Delta variants. PLOS ONE, 18(5), e0284173.
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5

Characterizing Netrin-1 Binding Kinetics

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Surface Plasmon Resonance analyses were carried out on a BIAcore T200 (GE Healthcare) instrument at 25 °C. Netrin-1 (Netrin-1-Fc; ProSci, #90-261) was immobilized on a Protein G sensor chip (GE Healthcare) through injection at a concentration of 30 μg/ml. Recombinant UNC5B-FL and UNC5B-Δ8 ectodomains analytes, prepared in PBS, 0.005% Tween 20, were injected at different concentrations (0–10 μM) using a 30 μl/min flow rate. Proteins were allowed to associate for 120 sec and to dissociate for 600 sec. Data were processed with the BIAcore BIAevaluation software v. 3.0.
Pradella D., Deflorian G., Pezzotta A., Di Matteo A., Belloni E., Campolungo D., Paradisi A., Bugatti M., Vermi W., Campioni M., Chiapparino A., Scietti L., Forneris F., Giampietro C., Volf N., Rehman M., Zacchigna S., Paronetto M.P., Pistocchi A., Eichmann A., Mehlen P, & Ghigna C. (2021). A ligand-insensitive UNC5B splicing isoform regulates angiogenesis by promoting apoptosis. Nature Communications, 12, 4872.
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