The gross motor activity of all the animals was assessed using the Opto-Varimex 4 Auto Track open-field Plexiglass chamber (Columbus Instruments, Columbus, OH). At the beginning of each session, the animals were placed in the middle of the Plexiglass chamber, and their subsequent movement and position were recorded continuously. For each animal, gross motor activity was assessed for three 30-min sessions. The sessions were conducted the day before METH treatment, immediately after the third METH injection (before the 2nd EpoD dose), and 24 h after the last METH injection (before the 3rd EpoD dose). Locomotor activity was calculated automatically from the raw beam-break data by the Opto-Varimex software.
Opto varimex 4 auto track open field plexiglass chamber
Manufactured by Columbus Instruments
The Opto-Varimex 4 Auto Track open-field Plexiglass chamber is a laboratory equipment designed for recording and analyzing animal movement and activity. It features a Plexiglass construction and automated tracking capabilities.
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2 protocols using opto varimex 4 auto track open field plexiglass chamber
1
Evaluating Gross Motor Activity in METH-Treated Animals
2
Methamphetamine Effects on Motor Activity
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Separate groups of PKO and WT rats were intraperitoneally (i.p.) injected with 2 mg/kg (free-base) of d-methamphetamine hydrochloride (Sigma-Aldrich, St. Louis, MO, USA) dissolved in sterile saline (0.9% NaCl). The gross motor activity of all the animals was assessed using the Opto-Varimex 4 AutoTrack open-field Plexiglass chamber (Columbus Instruments, Columbus, OH). At the beginning of each session, the animals were placed in the middle of the Plexiglass chamber, and their subsequent movement and position were recorded continuously. For each drug-naïve animal, motor activity was assessed during 30-min session. Activity of methamphetamine-treated rats was recorded for 100 min. Horizontal locomotor activity (distance travelled), and stereotypic movements (time spent) were calculated automatically from the raw beam-break data by the Opto-Varimex software.
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