Synthetic interstitial fluid (SIF) comprised (in mM) 107.8 NaCl, 26.2 NaCO3, 9.64 Na-gluconate, 7.6 sucrose, 5.05 glucose, 3.48 KCl, 1.67 NaH2PO4, 1.53 CaCl2 and 0.69 MgSO4, gassed with 95% O2 and 5% CO2 to obtain pH 7.4 [29 (link)]. Stock solutions of the TRPA1 antagonist HC030031, the TRPM8 antagonist AMTB hydrochloride and the TRPV4 antagonist RN 1734 (all purchased from Tocris Biosciences, Ellisville, USA) were made up in absolute methanol. Gadolinium(III) chloride, amiloride hydrochloride hydrate and ruthenium red (all purchased from Sigma-Aldrich, Steinheim, Germany) and the final dilutions were prepared by dissolving the stock solutions in SIF on the day of each experiment. The pH was adjusted to 7.4 by adding drops of HCl or NaOH.
Amtb hydrochloride
Manufactured by Bio-Techne
Sourced in United States, United Kingdom, Japan
AMTB hydrochloride is a chemical compound used as a research tool in scientific laboratories. It is a potent and selective antagonist of the transient receptor potential melastatin 8 (TRPM8) ion channel, which is involved in the regulation of temperature sensation and pain perception. The core function of AMTB hydrochloride is to inhibit the activity of TRPM8, allowing researchers to study the role of this channel in various biological processes.
Automatically generated - may contain errors
Lab products found in correlation
Amiloride hydrochloride hydrate, by Merck Group (1 mentions)
Ruthenium red, by Merck Group (1 mentions)
Hc 030031, by Bio-Techne (1 mentions)
Gadolinium 3 chloride, by Merck Group (1 mentions)
Rn1734, by Bio-Techne (1 mentions)
Chlorpromazine hydrochloride, by Merck Group (1 mentions)
Nortriptyline hydrochloride, by Merck Group (1 mentions)
Imipramine hydrochloride, by Merck Group (1 mentions)
Lacl3, by Merck Group (1 mentions)
Bapta am, by Thermo Fisher Scientific (1 mentions)
Thapsigargin, by Cayman Chemical (1 mentions)
Ethylene glycol tetraacetic acid (egta), by Merck Group (1 mentions)
Flufenamic acid, by Merck Group (1 mentions)
Penicillin streptomycin p s, by Thermo Fisher Scientific (1 mentions)
Icilin, by Bio-Techne (1 mentions)
L 168 049, by Bio-Techne (1 mentions)
Trizol reagent, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
D glucose, by Merck Group (1 mentions)
Ab3243, by Abcam (1 mentions)
4 protocols using amtb hydrochloride
1
Synthetic Interstitial Fluid Protocol
2
Cell Labeling with Fluorescent Conjugates
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R9-TAMRA (carboxytetramethylrhodamine), R9C(PEG2000)-TAMRA, R9(lysozyme)-TAMRA and R9(BSA)-TAMRA conjugates were custom-synthesized by GenScript. PEG2000 was conjugated to cysteine, BSA and lysozyme were conjugated to the C-terminus of the R9 peptide and TAMRA was conjugated to the N-terminus. R9-HiLyte was custom-synthesized by AnaSpec. Imipramine hydrochloride, nortriptyline hydrochloride, LaCl3, chlorpromazine hydrochloride, flufenamic acid, HC030013, AP18 and EGTA were purchased from Sigma. Thapsigargin was from Cayman Chemicals, Ruthenium Red was from EMD Biosciences and AMTB hydrochloride was from Tocris Bioscience. BAPTA/AM was purchased from Invitrogen. Calcium-sensitive fluorescent dye (Cal-520 AM) was purchased from AAT Bioquest.
3
Evaluation of Adipogenic Modulators
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Menthol (PubChem CID: 16666), icilin (PubChem CID: 161930), N-(3-Aminopropyl)−2-[(3-methylphenyl) methoxy] -N-(2-thienylmethyl) benzamide hydrochloride (AMTB, PubChem CID: 16095383) and D-glucose were purchased from Sigma Aldrich (Sigma Aldrich, St. Louis, MO, USA). Isopropyl alcohol, trizol reagent, fetal bovine serum (FBS), and penicillin/streptomycin (P/S) were purchased from invitrogen (Invitrogen, Camarillo, CA, USA). Mouse 3T3-L1 preadipocyte (3T3-L1 PA), Dulbecco's modified Eagle's medium (DMEM), maintenance media (MM) and differentiation medium (DM) were purchased from Zenbio (Zenbio Inc. Research Triangle Park, NC). L-168,049 (4-[3-(5-Bromo-2-propoxyphenyl)-5-(4-chlorophenyl)-1H-pyrrol-2-yl] pyridine; PubChem CID: 5311276) was purchased from Tocris Bioscience (Tocris Bioscience, Bristol, UK).
Chemical compounds studied in this article -Menthol; icilin; AMTB hydrochloride; L-168,049
4
Investigating TRPM8 and p21/Cip1 in Cells
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Antibody (Ab) specific to β-actin and siRNAs against p21/Cip1 and TRPM8 were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Icilin was obtained from Fujifilm Wako Pure Chemical Corporation (Osaka, Japan). Ab specific to TRPM8 (ab3243) was purchased from Abcam (Cambridge, UK). ECL TM anti-mouse IgG, horseradish peroxidase-linked whole antibody (from sheep) and ECL TM anti-rabbit IgG, horseradish peroxidase-linked whole antibody (from donkey) were purchased from GE Healthcare (Buckinghamshire, UK). AMTB hydrochloride was purchased from Tocris Bioscience (Tokyo, Japan) and BCTC from MedChemExpress (Tokyo, Japan). Human TRPM8 expression vector (pRP[Exp]-EGFP/ Puro-CAG>hTRPM8[NM_024080.4]) was purchased from Vector Builder Japan (Kanagawa, Japan).
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