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3 protocols using anisaldehyde

1

Phytochemical Identification and Quantification

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2,2-Diphenyl-1-picrylhydrazyl free radical (DPPH•), gallic acid (97%), stigmasterol, and ethyl acetate were purchased from Sigma-Aldrich (Munich, Germany). Acetic acid, and methanol were purchased from Merck (Darmstadt, Germany), n-hexane from BDH (Poole, England), anisaldehyde from ACROS Organics (New Jersey, USA), and Milli-Q (Millipore) purified water was used. HPTLC separations were performed on 20 × 10 cm normal phase Silica gel 60 F254 HPTLC glass plates (Merck, Darmstadt, Germany).
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2

Antioxidant Potential Evaluation

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All reagents used were analytical grade. Ethyl acetate, 2,2-di(4-tert-octylphenyl)-1-picrylhydrazyl (DPPH·) free radical, gallic acid (97%), caffeic acid (98%), rosmarinic acid (≥98%), and abietic acid (≥75%), were obtained from Sigma-Aldrich (Castle Hill, Australia). Acetic acid, ethanol, methanol, were from Merck (Darmstadt, Germany), and anisaldehyde was from ACROS organics (Fair Lawn, NJ, USA). Milli-Q (Millipore) water was used to prepare all aqueous solutions.
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3

Antioxidant Evaluation of Phytochemicals

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All reagents used were analytical grade. Ethyl acetate, 2,2-diphenyl-1-picrylhydrazyl (DPPH•) free radical, and gallic acid (97%) were obtained from Sigma-Aldrich (Castle Hill, Australia). Acetic acid, ethanol, and methanol were from Merck (Darmstadt, Germany), and anisaldehyde was from ACROS organics (New Jersey, USA). Milli-Q (Millipore) water was used to prepare all aqueous solutions. Aluminium chloride (reagent grade, 98%), 3-hydroxytyrosol (≥98%), maslinic acid (≥98%), oleanolic acid (≥97%), rosmarinic acid (≥98%), rutin hydrate (≥94%), and ursolic acid (≥90%) were purchased from Merck (Merck KGaA, Darmstadt, Germany).
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