Ifs 66

All reagents for synthesis of the complexes, i.e., chiral amino alcohols, salicylaldehyde derivatives, and vanadium(V) oxytripropoxide were purchased from Aldrich company. The other chemicals were obtained from local sources and used without further purification. Carlo Erba MOD 1106 instrument was used to perform elemental analyses. UV-Vis spectra were conducted using a Perkin-Elmer LAMBDA 18 spectrophotometer, and CD spectra were conducted using a Jasco J-815 spectropolarimeter. IR spectra were recorded on Bruker IFS 66 as KBr pellets. Bruker AVANCE III 700 MHz spectrometer was employed for all NMR spectra measurements using TMS as a reference and DMSO-d₆ as a solvent. The catalytic reactions progress was monitored on a Shimadzu GC-2025 gas chromatograph with an FID detector, and Zebron ZB-5 capillary column and Shimadzu GCMS-QP2010 SE equipment were used for the confirmation of the identities of all oxidation products. The absorbance of the cells during the MTT assay was measured at 570 nm and 660 nm (reference value) by an ASYS Hitech GmbH microplate reader. Microscopic images were taken at 1 × 4 magnification with an Olympus microscope.

All chemicals were purchased from commercial sources and used without further purification. Solvents were dried and distilled using conventional methods. Melting points were measured on Heiztisch Mikroskop—Polytherm A (Wagner & Munz, Germany). NMR spectra were performed on Agilent 400-MR DDR2 (¹H: 400 MHz, ¹³C: 100 MHz). Deuterated solvents used are indicated in each case. Chemical shifts (δ) are expressed in ppm and refer to the residual peak of the solvent or TMS as an internal standard; coupling constants (J) are in Hz. The mass analyses were performed using the ESI technique on a Q–TOF (Micromass) spectrometer. Elemental analyses were carried out on Perkin–Elmer 240, Elementar vario EL (Elementar, Germany) or Mitsubishi TOX–100 instruments. All samples were dried in the desiccator over P₂O₅ under vacuum (1 Torr) at 80 °C for 8 h. The IR spectra were measured on an FT–IR spectrometer Nicolet 740 or Bruker IFS66 spectrometers equipped with a heated Golden Gate Diamante ATR–Unit (SPECAC) in KBr. A total of 100 Scans for one spectrum were co–added at a spectral resolution of 4 cm⁻¹. The courses of the reactions were monitored using TLC aluminium sheets with Silica gel 60 F254 (Merck). The column chromatography was performed on Silica gel 60 (Merck). HPLC was performed on Büchi Pure 850 FlashPrep chromatography instrument using Prontosil, 150 × 20 mm, 5 μm column.

CHN analysis of CP/O300 nanocomposites was successfully conducted using a Carlo Erba 1106 (Carlo Erba, Milano, Italy) (combustion temperature of 1,030°C, atmosphere of oxygen). TEM images were obtained on a TEM125K (SELMI, Ukraine) microscope working at 100 kV. Amorphous carbon film which covered the copper grid was used as a carrier for samples. UV–vis spectra were measured on double beam spectrophotometer 4802 (UNICO, Fairfield, NJ, USA) with a resolution of 1 nm. Photoluminescence (PL) and Fourier transform infrared spectroscopy (FTIR) spectra were registered using LS55 (PerkinElmer, Waltham, MA, USA) and IFS-66 (Bruker, Karlsruhe, Germany) spectrophotometers. Kinetics of fluorescence decay of the studied materials was measured with the Edinburg Instruments TCSPC Fluorescence Spectrometer F900 (Edinburgh Instruments Ltd., West Lothian, UK) using different lasers for the sample excitation. The fluorescence spectra were corrected for the instrument sensitivity.

The IR spectra were recorded on the BRUKER IFS 66 spectrophotometer in a KBr pellet over the 4400–650 cm⁻¹ range.