The following antibodies were prepared for flow cytometry: PerCP-Cy5.5–conjugated anti-mouse CD4, PE-conjugated anti-mouse IL-17A, FITC-conjugated anti-mouse IFN-γ, PE-conjugated anti-mouse CD25 (BD Bioscience), and Alexa-647–conjugated anti-mouse Foxp3 (Biolegend). Spinal cord cells were stained by anti-CD4 and F4/80 antibodies (Biolegend). CD4+ T cells were cultured with 100 nM PMA (Sigma), 1 µM inonomycin (Sigma), and GolgiPlug (BD Bioscience) for 2 h at 37°C. Intracellular staining (IFN-γ, IL-17A, and Foxp3) was performed after permeation and fixation of cells by using either the Cytofix/Cytoperm intracellular staining kit (BD Bioscience; for IFN-γ and IL-17A) or the Foxp3/Transcription factor staining buffer set (Affymetrix; for Foxp3).
Percp cy5.5 conjugated anti mouse cd4
Manufactured by BD
PerCP-Cy5.5–conjugated anti-mouse CD4 is a fluorochrome-conjugated antibody that binds to the CD4 cell surface marker on mouse cells. It is designed for use in flow cytometry applications to identify and quantify CD4-positive cell populations.
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Lab products found in correlation
Golgiplug, by BD (1 mentions)
Foxp3 transcription factor staining buffer set, by Thermo Fisher Scientific (1 mentions)
Cytofix cytoperm intracellular staining kit, by BD (1 mentions)
Phorbol myristate acetate (pma), by Merck Group (1 mentions)
Pe conjugated anti mouse il 17a, by BD (1 mentions)
Pe conjugated anti mouse cd25, by BD (1 mentions)
Pe conjugated anti mouse cd25, by Thermo Fisher Scientific (1 mentions)
Pe conjugated anti mouse cd11b, by BD (1 mentions)
Fitc conjugated anti mouse cd11b, by BD (1 mentions)
Pe cy7 conjugated anti mouse cd3, by BioLegend (1 mentions)
Af 647 conjugated anti mouse ly6g, by BioLegend (1 mentions)
2 protocols using percp cy5.5 conjugated anti mouse cd4
1
Multiparametric Flow Cytometry Analysis of Immune Cells
2
Immune Cell Phenotyping in Tumor Microenvironment
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In order to evaluate the phenotype of different immune cell populations, cells derived from the spleen or tumor of vehicle- or AZD1480-treated mice were stained with the following antibodies: phycoerythrin (PE)-Cy7-conjugated anti-mouse CD3 (BioLegend), Alexa Fluor 700 (AF700)-conjugated anti-mouse CD4 (BD Biosciences), AF647-conjugated anti-mouse CD8 (BioLegend), Horizon V450-conjugated anti-mouse CD45 (BD Biosciences), peridinin chlorophyll protein (PerCP)-Cy5.5-conjugated anti-mouse CD4 (BD Biosciences), PE-conjugated anti-mouse CD25 (eBioscience), AF700-conjugated anti-mouse CD127 (eBioscience), AF647-conjugated anti-mouse CD11c (BioLegend), PE-conjugated anti-mouse CD11b (BD Biosciences), fluorescein isothiocyanate (FITC)-conjugated anti-mouse CD86 (BD Biosciences), allophycocyanin (APC)-H7-conjugated anti-mouse CD80 (BD Biosciences), FITC-conjugated anti-mouse CD11b (BD Biosciences), AF647-conjugated anti-mouse Ly6G (BioLegend) and PECy7-conjugated anti-mouse Ly6C (BioLegend).
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