Mk118

Manufactured by Takara Bio

Sourced in Japan

The MK118 is a laboratory equipment designed for automated pipetting and liquid handling. It features precise volume control, programmable liquid handling protocols, and compatibility with a wide range of labware. The core function of the MK118 is to perform accurate and reproducible liquid transfers, enabling efficient sample preparation and assay workflows.

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Lab products found in correlation

Mk111, by Takara Bio (3 mentions) Cobas e601, by Roche (1 mentions) Beckman au5800 automatic biochemical analyzer, by Beckman Coulter (1 mentions) Advia centaur, by Siemens (1 mentions) Tr15421, by Thermo Fisher Scientific (1 mentions) 80 insrt e01, by ALPCO (1 mentions)

Spelling variants of this product

Glu-osteocalcin Enzyme Immuno Assay (EIA) Kit MK118 (5 citations)

4 protocols using mk118

Bone Turnover Markers Serum Analysis

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ELISA kits were used to measure the serum levels of human bone Alkaline Phosphatase (bALP; Cusabio, CSB-E09033h, Houston, TX, USA), procollagen I N-terminal Peptide (PINP; Cusabio, CSB-E11226h), carboxyterminal propeptide of type I procollagen (PICP; Cusabio, CSB-E08079h), cross linked N-telopeptide of type I collagen (NTX; Cusabio, CSB-E09233h), and cross linked C-telopeptide of type I collagen (CTX; Novusbio, NBP2-69073, Centennial, CO, USA).
EIA kits were used to measure serum levels of human osteocalcin in the undercaboxylated (unOC) and carboxylated (cOC) form (Takara, Shiga, Japan, MK-118 and MK-111, respectively).

Mandatori D., Penolazzi L., Pelusi L., Lambertini E., Michelucci F., Porreca A., Cerritelli P., Pipino C., Di Iorio A., Bruni D., Di Nicola M., Buda R., Piva R, & Pandolfi A. (2021). Three-Dimensional Co-Culture System of Human Osteoblasts and Osteoclast Precursors from Osteoporotic Patients as an Innovative Model to Study the Role of Nutrients: Focus on Vitamin K2. Nutrients, 13(8), 2823.

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Bovine Osteocalcin Concentration Analysis

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Cows were randomly selected within KET and CON groups, respectively, for the analysis of cOC (n = 16 in KET and n = 15 in CON) and ucOC (n = 17 in KET and n = 18 in CON) concentrations, and plasma samples from BW–1 and BW1 were used. The analysis was performed using cOC and ucOC ELISA kits (MK111 and MK118, Takara, Shiga, Japan), and the intra-and inter-assay coefficients of variation (CV) were 4.3 and 3.6% for cOC and 5.9 and 7.8% for ucOC, respectively.

Choi W., Ro Y., Choe E., Hong L., Kim D., Kim S., Yoon I, & Kim D. (2023). Comparison of prepartum blood parameters in dairy cows with postpartum ketosis and new risk prediction candidates. Frontiers in Veterinary Science, 10, 1161596.

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Serum Biomarkers Assessment Protocol

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Blood sample were harvested in the morning after at least 8 hours fasting. Serum was gathered after centrifugation at 3,000 r/min for 10 minutes. Serum ucOC were assayed by ELISA (MK118, Takara Bio Inc, Japan), with the intra assay coe cient of variation of 4.58%-6.66% and the inter assay coe cient of variation of 5.67%-9.87%. Serum cOC were measured by ELISA (MK111, Takara Bio Inc, Japan), with the intra assay coe cient of variation of 3.3%-4.8%, the inter assay coe cient of variation of 0.7%-2.4%.
Serum calcium (Ca), phosphorus (Pi), and alkaline phosphatase (ALP) measurements were performed by a Beckman Automatic Biochemical Analyzer (AU5800, Beckman Coulter, Indianapolis, IN, USA). Serum OC, β-isomer of C-terminal telopeptides of type I collagen (β-CTX), N-terminal prepeptide of type 1 procollagen (P1NP) were measured by electrochemiluminescence immunoassay (Roche Cobas e601, Mannheim, Germany). Parathyroid hormone (PTH) was measured using chemiluminescence (Siemens ADVIA Centaur, Munich, Germany). 25hydroxyvitamin D (25OHD) was measured by electrochemiluminescence immunoassay (Roche Cobas e601, Mannheim, Germany). Creatinine (Cr) was measured by an automated Roche electrochemiluminescence system (E170; Roche Diagnostics, Basel, Switzerland). All measurements were completed by central laboratory of PUMCH.

Liu S., Pang Q., Guan W., Yu F., Wang O., Li M., Xing X., Yu W., Jiang Y, & Xia W. (2024). Association of serum osteocalcin with bone microarchitecture and muscle mass in Beijing community-dwelling postmenopausal women. Endocrine, 84(1).

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Hormone Profiling in Euthanized Rats

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Blood was collected from the inferior vena cava at the time of euthanasia, and plasma was stored at −80°C until analysis. All analyses were performed in duplicate. Plasma insulin, and undercarboxylated osteocalcin were measured by ELISA (80-INSRT-E01 ALPCO, Salem, NH and MK118 Takara Bio USA, Mountain View, CA respectively); plasma glucose was measured using a colorimetric assay (TR15421, Thermo Fisher Scientific, Waltham, MA). Follicle stimulating hormone (FSH) was measured by MILLIPLEX map Rat Pituitary Panel kit, EMD Millipore product # RPTMAG-86K.

Sherk V.D., Jackman M.R., Higgins J.A., Giles E.D., Foright R.M., Presby D.M., Carpenter R.D., Johnson G.C., Oljira R., Houck J.A, & MacLean P.S. (2019). Impact of Exercise and Activity on Weight Regain and Musculoskeletal Health PostOVX. Medicine and science in sports and exercise, 51(12), 2465-2473.

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