Chicken anti gfap ab4674

Manufactured by Abcam

ab4674 is a chicken polyclonal antibody that recognizes Glial Fibrillary Acidic Protein (GFAP), an intermediate filament protein found in astrocytes and other glial cells in the central nervous system.

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Lab products found in correlation

Axiocam digital camera, by Zeiss (2 mentions) Microm ks 34, by Thermo Fisher Scientific (1 mentions) Zen 2012, by Zeiss (1 mentions) Rabbit anti atf3, by Santa Cruz Biotechnology (1 mentions) Rabbit anti iba1 019 19741, by Fujifilm (1 mentions) Neurotrace, by Thermo Fisher Scientific (1 mentions) Axio imager z1 microscope, by Zeiss (1 mentions) Vectashield, by Vector Laboratories (1 mentions) Mouse anti flag f1804, by Merck Group (1 mentions) Alexa fluor 488 or 555 secondary antibodies, by Thermo Fisher Scientific (1 mentions)

2 protocols using chicken anti gfap ab4674

Mouse Brain Immunofluorescence Staining

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For mouse brain staining, brains were dissected and fixed in 4% paraformaldehyde for 1 hr on ice followed by immersion in 20% sucrose overnight at 4°C. Brains were then sectioned using a freezing stage Microtome (Microm KS 34, Thermo Scientific) and spread out on glass coverslips for immunostaining. The antibodies were used at the following concentrations: mouse anti-AnkG (N106/36, N106/20) (UC Davis/NIH), 1:200; rabbit anti-ATF3 (Santa Cruz) (RRID:AB_2058590), 1:500; chicken anti-GFP (ab13970, abcam) (RRID:AB_300798), 1:1000; rabbit anti-Iba1 (019–19741, Wako), 1:250; chicken anti-GFAP (ab4674, abcam) (RRID:AB_304558), 1:500; NeuroTrace (Thermo Scientific), 1:200; Alexa 488-, Cy3-, or Cy5-conjugated secondary antibodies (Jackson ImmunoResearch), 1:250. Samples were then mounted in Vectashield (Vector Laboratories) before being analyzed under a confocal microscope. The images were obtained with an Axio-imager Z1 microscope (Carl Zeiss) fitted with an AxioCam digital camera (Carl Zeiss). The images were analyzed by ZEN 2012 (Carl Zeiss).

Chen K., Ho T.S., Lin G., Tan K.L., Rasband M.N, & Bellen H.J. (2016). Loss of Frataxin activates the iron/sphingolipid/PDK1/Mef2 pathway in mammals. eLife, 5, e20732.

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Immunostaining for Brain Histology

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Brain histology was analyzed using brain sections and immunostaining. Mice under anesthesia were transcardially perfused with 4% PFA and brains were removed, post-fixed in 4% PFA for 24 hours, and infiltrated with 20–30% sucrose. Brain sections (20 μm-thick) were cut using cryostat, blocked with serum of appropriate species, treated with primary antibodies: mouse antic-Fos (sc-166940, Santa Cruz), mouse anti-GFP (A-11120, Thermo Fisher), mouse anti-FLAG (F1804, Sigma) and chicken anti-GFAP (ab4674, Abcam), subsequently reacted with Alexa Fluor 488 or 555 secondary antibodies (Invitrogen). Naïve IgGs of appropriate species were used as negative controls. DAPI staining was used to reveal all cells in the section. Images were taken using a confocal microscope.

Zhang Y., Reichel J.M., Han C., Zuniga-Hertz J.P, & Cai D. (2017). Astrocytic process plasticity and IKKβ/NF-κB in central control of blood glucose, blood pressure and body weight. Cell metabolism, 25(5), 1091-1102.e4.

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