Dna iq casework pro kit for maxwell 16

The samples were processed applying the standard laboratory protocol in order to perform the comparative analysis. The Genomic DNA extraction required a manual lysis phase followed by automated extraction through “Maxwell® 16 MDx Instrument” (Promega, WI, USA) in association with the kit “DNA IQ™ Casework Pro Kit for Maxwell® 16” (Promega, WI, USA) according to the manufacturer’s instruction. Quantifiler® Trio DNA Quantification Kit (Thermo Fisher Scientific, Waltham, MA, USA) was used to evaluate the concentration and the quality of the purified DNA followed by analysis through the software “HID Real-Time PCR Analysis Software” according to the instructions provided by the kit. The Quantifiler™ Trio DNA Quantification Kit simultaneously assesses DNA concentration and degradation by detecting two human multicopy autosomal targets: a small amplicon of 80 bp in length and a large amplicon of 214 bp size. Using the ratio between the concentrations of both amplicons (short/large) the level of degradation could be measured. Thus, the larger target is more likely to be affected by degradation of the DNA template than the shorter autosomal target. When DNA is degraded, the concentration of DNA detected with the degradation target is less than the concentration detected with the autosomal target.