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Lpl activity assay kit

Manufactured by Merck Group
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The LPL activity assay kit is a laboratory instrument designed to measure the activity of the enzyme lipoprotein lipase (LPL). LPL is involved in the metabolism of lipids and plays a role in the regulation of blood lipid levels. The assay kit provides a standardized method for quantifying LPL activity in various biological samples.

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Lipase activity assay kit 3, by Merck Group (1 mentions)

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Activity Assay Kits

2 protocols using lpl activity assay kit

1

Lipoprotein Lipase Activity Assay

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LPL activity was determined with a 50 μl aliquot of culture medium after 30 min of treatment with 50 units of heparin using an LPL activity assay kit (Sigma) according to the manufacturer's instructions. Intracellular total lipase activity was measured using a lipase activity assay kit III (Sigma).
Barilla S., Liang N., Mileti E., Ballaire R., Lhomme M., Ponnaiah M., Lemoine S., Soprani A., Gautier J.F., Amri E.Z., Le Goff W., Venteclef N, & Treuter E. (2020). Loss of G protein pathway suppressor 2 in human adipocytes triggers lipid remodeling by upregulating ATP binding cassette subfamily G member 1. Molecular Metabolism, 42, 101066.
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2

Heparin-induced Lipoprotein Lipase Activity

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We measured the pre-heparin and post-heparin LPL and HL activity. Blood samples were collected from the proband and his brother (Ⅱ-3) after an overnight fast. Their blood was collected again 15 min after an intravenous bolus injection of heparin (100 IU/kg). His brother was set as a normal control for triglyceride. LPL and HL activity assays of other family members were not performed because they refused to receive heparin. LPL and HL activities were measured as previously described24 (link)). LPL and HL activities were detected by LPL Activity Assay Kit (Sigma, Roar Biomedical Inc.). Each assay was performed in three independent tests. The data were analyzed by unpaired two-tailed tests using Graph Pad Prism V.5 software (V.5.0).
Liu Y., Xu J., Tao W., Yu R, & Zhang X. (2019). A Compound Heterozygous Mutation of Lipase Maturation Factor 1 is Responsible for Hypertriglyceridemia of a Patient. Journal of Atherosclerosis and Thrombosis, 26(2), 136-144.
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