To evaluate the condition of the skin barrier, patients were investigated instrumentally for TEWL, using a Tewameter® TM 300 (Courage + Khazaka electronic GmbH, Cologne, Germany) under the manufacturer’s instructions. The condition of all subjects was first stabilized for 15–20 min, in a climate- and humidity-controlled room, with an ambient temperature range of 21–25°C and mean relative humidity range of 50–60%. Two topical comparative plaques of homologous anatomic positions and severity on each side were measured at each visit at weeks 0, 2 and 4. Additionally, five healthy volunteers were recruited as the control.
Tewameter tm300
Manufactured by Courage + Khazaka
Sourced in Germany
The Tewameter TM300 is a device designed to measure transepidermal water loss (TEWL). It is a scientific instrument used to quantify the water evaporation rate from the skin surface, which is an important indicator of skin barrier function and hydration levels.
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Lab products found in correlation
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Skin ph meter ph 905, by Courage + Khazaka (6 mentions)
Cutometer mpa 580, by Courage + Khazaka (6 mentions)
Cutometer dual mpa 580, by Courage + Khazaka (4 mentions)
Corneometer 825, by Courage + Khazaka (3 mentions)
Mexameter mx18, by Courage + Khazaka (3 mentions)
Scotch magic tape, by 3M (2 mentions)
Mpa adapter, by Courage + Khazaka (2 mentions)
Visioscan vc 98 usb, by Courage + Khazaka (2 mentions)
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Visia complexion analysis, by Canfield Scientific (1 mentions)
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Cellulose acetate membrane, by Sartorius (1 mentions)
Visioface 1000d, by Courage + Khazaka (1 mentions)
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Ck multi probe software, by Courage + Khazaka (1 mentions)
132 protocols using tewameter tm300
1
Skin Barrier Evaluation via TEWL Measurement
2
Skin Barrier Assessment and Epidermal Irritation
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TEWL was measured using the Tewameter TM300 (probe 06030433, Courage & Khazaka, Cologne, Germany). Tape-stripping consisted of 3 repetitions of adhesive tape application (Scotch Magic tape, 3M, St. Paul, MN) performed one week following hair removal. Epidermal abrasion was induced by twice repeated shaving of ventral skin (Personna American Safety Razor Co., Verona, VA). Ear thickness following a single application of TPA (Sigma, 0.5 nmoles) was measured using an engineer’s micrometer (Mitutoyo 7301). Epidermal hypertrophy was induced by TPA (20 nmoles) application 2x/wk for 4 wks. Histologic examination and measurement of minimal epidermal thickness was performed as previously described (Lewis et al., 2014 (link)),
3
Skin Barrier Assessment and Epidermal Irritation
4
Skin Condition Assessment Protocol
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Clinical evaluations of skin symptoms were performed at 0, 6, 12, and 18 months by a dermatologist. Regarding skin surface conditions, stratum corneum hydration, transepidermal water loss (TEWL), and skin color tone uniformity were measured non-invasively at 0 and 18 months. Stratum corneum hydration and TEWL on the cheek were measured non-invasively (Corneometer® CM825 and Tewameter® TM 300; Courage+Khazaka electronic GmbH, Cologne, Germany). The subjects’ faces were photographed using a digital image analysis system (Visia® Complexion Analysis; Canfield Scientific, Inc., Fairfield, NJ, USA). The number of pigmentation spots and wrinkles on the face were counted using the pictures. The skin color tone uniformity was assessed by detecting uneven skin tone area of the face. The uneven skin tone area was defined as significantly different color compared to peripheral skin. The photographs were analyzed in a pixel unit and the pixels with significantly different color compared to peripheral skin were counted as uneven skin tone. The areas of the uneven skin tone were calculated using the image analysis system.
5
Measuring Skin Barrier Function via TEWL
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Transepidermal water loss (TEWL) was used as a permeability marker to indicate skin barrier function and was measured using the Tewameter® TM300 (Courage + Khazaka Electronic GmbH, Cologne, Germany) 10 weeks after the experiment started. Values were recorded once the responses stabilized, typically 10 s after placing the probe on the skin. TEWL was measured as the average of three iterations in each test area.
6
Measuring Skin Barrier and Hydration
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TEWL and the skin hydration levels of the dorsal skin were respectively measured using a Tewameter® TM300 and Corneometer® CM825 (Courage+Khazaka Electronic GmbH, Cologne, Germany) on days zero, seven, 14, and 21, according to previously described methods7 (link).
7
Skin Barrier Evaluation in Elderly
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TEWL is defined as the flux density of water diffusing from the dermis and epidermis through the stratum corneum to the skin surface.5 It was measured using Tewameter®TM300 (Courage+Khazaka), which is the gold standard of TEWL measurement. The measurements were conducted for 30 s, and the average value was employed. These measurements are founded on the principle of evaluating the water vapor pressure gradient just above the skin surface and are represented in units of g/m2/h.8 As with the SCH measurement method, the measurement error was minimized.
A previous study on older residents reported that a higher ODS was associated with lower TEWL values.11 Higher TEWL values are often associated with skin barrier impairments in adults. However, the skin of the elderly is generally dry due to decreased sebum production and hypertrophy of keratinocytes, and TEWL tends to be low.17 Therefore, this outcome measure was used as external criteria to verify concurrent validity of the outcome of the ODS‐J.
A previous study on older residents reported that a higher ODS was associated with lower TEWL values.
8
Skin Barrier Evaluation in Mice
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TEWL values and the skin hydration index of the dorsal skin of the mice were measured at 20-22℃ and 40％ relative humidity, on days 0, 7, 11, and 14 of the experimental period using a Tewameter TM 300 and Corneometer CM 825 (Courage Khazaka electronic GmbH, Cologne, Germany) , respectively.
9
Skin Hydration and Barrier Function Assessment
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Ten female volunteers with healthy skin between 25 and 35 years old participated in the study. The study was approved by the Ethics Committee of the University of Barcelona (reference number: IRB00003099; date: 20 March 2018) following the guidelines of the Declaration of Helsinki [29 (link)] and all volunteers signed written informed consent forms. Skin-care cosmetics were not permitted on the test areas for two days prior to the study. Volunteers stayed in the test room for at least 30 min prior to the measurements. Measurements were performed before applying the gel (to establish the baseline readings) and 15 min, 1 h and 2 h after the application of 0.5 g on the flexor side of the left forearm.
Trans epidermal water loss (TEWL), referring to the total amount of water vapor lost through the skin was measured by a Tewameter® TM 300 (Courage-Khazaka electronic GmbH, Cologne, Germany). The stratum corneum hydration (SCH) was determined using a Corneometer® CM 825 (Courage-Khazaka electronic GmbH). Measurements were performed according to international guidelines [30 (link)].
Trans epidermal water loss (TEWL), referring to the total amount of water vapor lost through the skin was measured by a Tewameter® TM 300 (Courage-Khazaka electronic GmbH, Cologne, Germany). The stratum corneum hydration (SCH) was determined using a Corneometer® CM 825 (Courage-Khazaka electronic GmbH). Measurements were performed according to international guidelines [30 (link)].
10
Neonatal Skin Barrier Permeability
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TEWL rate was assessed by Tewameter TM300 (Courage + Khazaka electronic GmbH as described in Quiroz et al., 2020 (link)). Basically neonates were sacrificed and their back skin was dissected and immediately spread over a clean, smooth surface. Over four TEWL measurements were collected on each piece of fully acclimatized skin. The values reported by the instrument were then normalized to the average values from the corresponding control samples to get relative water loss rate.
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