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Vevo 770 high resolution in vivo micro imaging system

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The Vevo 770 High-Resolution In Vivo Micro-Imaging System is a laboratory equipment product designed for high-resolution imaging of small animals and tissues. It utilizes high-frequency ultrasound technology to capture detailed images and measurements of physiological processes in real-time.

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Lab products found in correlation

Rmv 707b, by Fujifilm (2 mentions) Ultrasound transmission gel, by Parker Laboratories (2 mentions) Rmv707b scan head, by Fujifilm (2 mentions) Vevo mouse handling table, by Fujifilm (1 mentions) Isofluorane, by Henry Schein (1 mentions) Vevo 770, by Fujifilm (1 mentions) Ultra sensitive mouse cardiac troponin 1 elisa kit, by Life Diagnostics (1 mentions)

19 protocols using vevo 770 high resolution in vivo micro imaging system

1

Echocardiographic Evaluation in Mice

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We performed echocardiography under inhalant isoflurane anesthesia 1 week prior to sacrifice using a Vevo 770 High-Resolution In vivo Micro-Imaging System (VisualSonics, Toronto, Canada). We used the parasternal short- and long-axis views to obtain 2-dimensional and M-mode images. We acquired at least 10 independent cardiac cycles for each experiment.
Dussold C., Gerber C., White S., Wang X., Qi L., Francis C., Capella M., Courbon G., Wang J., Li C., Feng J.Q., Isakova T., Wolf M., David V, & Martin A. (2019). DMP1 prevents osteocyte alterations, FGF23 elevation and left ventricular hypertrophy in mice with chronic kidney disease. Bone Research, 7, 12.
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2

Echocardiographic Assessment of Cardiac Function in Mice

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Echo analysis was performed in mice that were anaesthetized with gaseous isoflurane. Anaesthetized mice were analyzed via a Vevo 770 high-resolution in vivo micro-imaging system (VisualSonics, inc) with a 30 MHz RMV-707B ultrasonic probe. Left ventricle anterior wall (LVAW), left ventricle posterior wall (LVPW), left ventricle internal dimension (LVID), and left ventricle volume (LV Vol) of systole and diastole were determined on M-mode images. Equations for calculation of ejection fraction (EF) and fractional shortening (FS) are as follows, EF% = [(LV Vol;d - LV Vol;s)/LV Vol;d] x 100%, and FS% = [(LVID;d – LVID;s)/LVID;d] x 100%.
Zhu S., Quan C., Wang R., Liang D., Su S., Rong P., Zhou K., Yang X., Chen Q., Li M., Du Q., Zhang J., Fang L., Wang H.Y, & Chen S. (2022). The RalGAPα1–RalA signal module protects cardiac function through regulating calcium homeostasis. Nature Communications, 13, 4278.
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3

Ultrasound Analysis of Fetal Hemodynamics

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Umbilical artery blood velocity and fetal heart rate were determined using a Vevo 770 High-Resolution In Vivo Micro-Imaging System (VisualSonics) fitted with a 40 MHz transducer. Mice were anesthetized with 3% isoflurane at 2 L O2/min for the duration of the scan. Mice were kept warm on a heated platform (37°C) that was connected to a temperature/physiology monitor (Indus Instruments). Abdominal fur was removed using over-the-counter hair remover (Nair, Church & Dwight Co.). Ultrasound Transmission Gel (Parker Laboratories) was applied to the abdominal area. B-mode ultrasound was used to structurally identify placentas, fetuses, and umbilical arteries. The power-Doppler mode was used to monitor blood flow velocities and fetal heart rates. Images were saved as static images or cine loops and quantified using the vendor-provided software.
Braun A.E., Carpentier P.A., Babineau B.A., Narayan A.R., Kielhold M.L., Moon H.M., Shankar A., Su J., Saravanapandian V., Haditsch U, & Palmer T.D. (2019). “Females Are Not Just ‘Protected’ Males”: Sex-Specific Vulnerabilities in Placenta and Brain after Prenatal Immune Disruption. eNeuro, 6(6), ENEURO.0358-19.2019.
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4

4T1 Mammary Tumor Metastasis Model

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Anesthetized mice (2% isoflurane) with thoracic cavity hair removed with chemical depilatory were imaged using a Vevo 770 High-Resolution In Vivo Micro-Imaging System (Visual Sonics, Toronto, ON, Canada) and a 35 MHz mechanical transducer. 5,000 4T1 isogenic mammary tumor cells/100 µl PBS were loaded in a 1 ml syringe with a 30-gauge 1” needle and the needle tip rinsed with sterile saline to remove external tumor cells. Under ultrasound image guidance, the needle was placed into the left ventricle, tumor cells injected, and needle held within the heart for 4–6 seconds to ensure tumor cells entered the circulation. Nulliparous and involution day 2 mice were alternately injected. Mice were weighed daily and euthanized in a rolling study design, in pairs, one/group, upon weight loss (10–15% of body weight). All mice were euthanized 16–24 days post-injection (Nullip, n=24; Inv2, n=25). Presence of 4T1 tumor cells in liver, lung, bone, and brain was determined using clonogenic assays (25 ). Mice were excluded if thoracic tumors were evident.
Goddard E.T., Hill R.C., Nemkov T., D’Alessandro A., Hansen K.C., Maller O., Mongoue-Tchokote S., Mori M., Partridge A.H., Borges V.F, & Schedin P. (2016). The rodent liver undergoes weaning-induced involution and supports breast cancer metastasis. Cancer discovery, 7(2), 177-187.
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5

Imaging Thyroid Tumor Volume

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Mice were anesthetized with isoflurane with 1%O2. Thyroid tumors were imaged using Vevo-770 High-Resolution In Vivo Micro-Imaging System (VisualSonics). Volume was calculated by manually tracing the margin of the tumor every 250 μm using the instrument software.
Garcia-Rendueles M.E., Krishnamoorthy G., Saqcena M., Acuña-Ruiz A., Revilla G., de Stanchina E., Knauf J.A., Lester R., Xu B., Ghossein R.A, & Fagin J.A. (2022). Yap governs a lineage-specific neuregulin1 pathway-driven adaptive resistance to RAF kinase inhibitors. Molecular Cancer, 21, 213.
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6

Echocardiography in Anesthetized Mice

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Mice were anesthetized with an isoflurane vaporizer (VetEquip) and attached to ECG leads on a Vevo Mouse Handling Table (VisualSonics Inc.). Chest hair was removed with depilatory cream. Transthoracic echocardiography was performed with heart rate approximately 500–550 bpm using a Vevo 770 High-Resolution In Vivo Micro-Imaging System and RMV 707B scan-head (VisualSonics Inc.). A single, experienced echocardiographer blinded to genotype acquired the images. Parasternal 2D images and M-mode images were acquired to assess LV chamber size and fractional shortening (calculated as FS = [LVEDD-LVESD]/LVEDD).
Burke M.A., Chang S., Wakimoto H., Gorham J.M., Conner D.A., Christodoulou D.C., Parfenov M.G., DePalma S.R., Eminaga S., Konno T., Seidman J.G, & Seidman C.E. (2016). Molecular profiling of dilated cardiomyopathy that progresses to heart failure. JCI Insight, 1(6), e86898.
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7

Thyroid Tumor Imaging in Mice

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Mice were anesthetized by inhalation of isoflurane. Thyroid tumors were imaged using Vevo 770 High-Resolution In Vivo Micro-Imaging System (VisualSonics). Aqueous ultrasonic gel was applied to the denuded skin overlying the thyroid gland prior to placement of the ultrasonic transducer. Volume was calculated by manually tracing the margin of the tumor every 250 μm using the instrument software. Genotypes were blinded for volume analysis. Volume calculation for all tumors was performed by the same person (IL) to avoid inter-operator bias.
Landa I., Thornton C.E., Xu B., Haase J., Krishnamoorthy G.P., Hao J., Knauf J.A., Herbert Z.T., Blasco M.A., Ghossein R, & Fagin J.A. (2023). Telomerase reactivation induces progression of mouse BrafV600E-driven thyroid cancers without telomere lengthening. bioRxiv.
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8

Echocardiography of Anesthetized Mice

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Mice were anaesthetized with gaseous isoflurane as afore-mentioned. Echocardiography was performed on anaesthetized mice using a Vevo 770 high-resolution in vivo micro-imaging system (VisualSonics, inc) with a 30MHz RMV-707B ultrasonic probe as previously described [21 (link)]. Briefly, mice were shaved on their chests, and then placed on a heating pad in a supine position with their chests covered with ultrasound transmission gel. The ultrasonic probe was immobilized with a 90° angle between the probe and the heart to collect M-mode pictures. The following parameters were measured on the M-mode tracing and averaged from 6 cardiac cycles: left ventricle anterior wall (LVAW), left ventricle posterior wall (LVPW), left ventricle internal dimension (LVID) of systole and diastole. The calculation of ejection fraction (EF) formula is EF% = [(LV Vol;d—LV Vol;s)] x 100%, and fraction shortening (FS) is calculated as FS% = [(LVID;d—LVID;s)/LVID;d] x 100%.
Quan C., Xie B., Wang H.Y, & Chen S. (2015). PKB-Mediated Thr649 Phosphorylation of AS160/TBC1D4 Regulates the R-Wave Amplitude in the Heart. PLoS ONE, 10(4), e0124491.
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9

Thyroid Tumor Imaging in Mice

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Mice were anesthetized by inhalation with isofluorane (Henry Schein, Melville, NY) with 1% O2. Thyroid tumors were imaged using Vevo 770 High-Resolution In Vivo Micro-Imaging System (VisualSonics, Toronto, CA). Aqueous ultrasonic gel was applied to the denuded skin overlying the thyroid gland prior to placement of the ultrasound transducer. Volume was calculated by manually tracing the margin of the tumor every 250 microns using the instrument software.
Saqcena M., Leandro-Garcia L.J., Maag J.L., Tchekmedyian V., Krishnamoorthy G.P., Tamarapu P.P., Tiedje V., Reuter V., Knauf J.A., de Stanchina E., Xu B., Liao X.H., Refetoff S., Ghossein R., Chi P., Ho A.L., Koche R.P, & Fagin J.A. (2020). SWI/SNF complex mutations promote thyroid tumor progression and insensitivity to redifferentiation therapies.. Cancer discovery, 11(5), 1158-1175.
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10

Echocardiographic Assessment of Cardiac Function

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Echocardiography was performed by the Vevo 770 High-Resolution In Vivo Micro-Imaging System (VisualSonics, Vevo-770) before and 24 h after LPS stimulation. After mice were anesthetized by isoflurane (RWD, R510-22-4) inhalation through a mask, echocardiography parameters were measured under the long-axis M-mode with heart rate over 350 beats per minute, including left ventricular internal end-diastolic diameter (LVID, d), left ventricular internal end-systolic diameter (LVID, s), fractional shortening (FS), ejection fraction (EF) and other parameters.
Ni L., Lin B., Shen M., Li C., Hu L., Fu F., Chen L., Yang J, & Shi D. (2022). PKM2 deficiency exacerbates gram-negative sepsis-induced cardiomyopathy via disrupting cardiac calcium homeostasis. Cell Death Discovery, 8, 496.
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