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Clostridium scindens

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Clostridium scindens is an anaerobic bacterium that can be used for laboratory research. It is a Gram-positive, spore-forming rod-shaped bacterium.

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Lab products found in correlation

Ruminococcus gnavus, by American Type Culture Collection (1 mentions) Bacteroides uniformis, by American Type Culture Collection (1 mentions) Bacteroides caccae, by American Type Culture Collection (1 mentions) Bacteroides vulgatus, by American Type Culture Collection (1 mentions) Parabacteroides distasonis, by American Type Culture Collection (1 mentions) Bacteroides ovatus, by American Type Culture Collection (1 mentions)

2 protocols using clostridium scindens

1

Gut Microbiome Cultivation Protocol

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Ruminococcus gnavus (29149), Coprococcus comes (27758), Dorea formicigenerans (27755), Clostridium nexile (27757), Clostridium scindens (35704), and Clostridium ramosum (25582) were purchased from the ATCC. The UC Lachnospiraceae strain was isolated as described below. Listeria monocytogenes was a gift from Bana Jabri and Lactobacillus rhamnosus was a gift from John Alverdy. Microbial strains were grown on Schaedler blood agar (BD) or chopped meat carbohydrate broth (BD) in an anaerobic jar at 37°C with the exception of Candidatus Arthromitus (SFB), which was propagated in monocolonized mice in gnotobiotic isolators and analyzed by staining of fecal samples.
Bunker J.J., Drees C., Watson A.R., Plunkett C.H., Nagler C.R., Schneewind O., Eren A.M, & Bendelac A. (2019). B cell superantigens in the human intestinal microbiota. Science translational medicine, 11(507), eaau9356.
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2

Culturing Synthetic Gut Microbiome

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Synthetic community strains were cultured in pre-reduced Mega Medium15 (link) or on EG Agar29 at 37 °C in an anaerobic chamber (Coy Laboratories, Grass Lake, MI, USA) with an atmosphere of 5% hydrogen, 10% CO2 and 85% N2. Synthetic community strains included Bacteroides uniformis ATCC 8492, Bacteroides vulgatus ATCC 8482, Bacteroides thetaiotaomicron VPI-5482, Bacteroides caccae ATCC 43185, Bacteroides ovatus ATCC 8483, Parabacteroides distasonis ATCC 8503, Eubacterium rectale ATCC 33656, Dorea longicatena DSM 13814, Ruminococcus torques ATCC 27756, and Clostridium scindens ATCC 35704. All strains were obtained from the American Type Culture Collection (ATCC), except D. longicatena DSM 13814, which was obtained from The Leibniz Institute DSMZ (Braunschweig, Germany).
Wahlström A., Brumbaugh A., Sjöland W., Olsson L., Wu H., Henricsson M., Lundqvist A., Makki K., Hazen S.L., Bergström G., Marschall H.U., Fischbach M.A, & Bäckhed F. (2024). Production of deoxycholic acid by low-abundant microbial species is associated with impaired glucose metabolism. Nature Communications, 15, 4276.
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