Dts1060

Manufactured by Malvern Panalytical

Sourced in United Kingdom

The DTS1060 is a differential scanning calorimeter (DSC) instrument manufactured by Malvern Panalytical. It is designed to measure the thermal properties of materials, including phase transitions, heat capacity, and thermal stability. The instrument uses a controlled temperature program to heat or cool a sample and a reference material, and measures the difference in heat flow between the two.

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Lab products found in correlation

39 protocols using dts1060

Characterization of Polymer Nanoparticles with Cas9 and gDonor

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PAsp(DET) nanoparticles with gDonor and Cas9 were formulated as described above. The polymer nanoparticles were centrifuged at 17,000 g for 10 min, and the supernatant and pellet were collected. Each sample was mixed with a 100 µg of heparin for particle dissociation. The collected supernatant and pellets were run on a gel, and analyzed for the Cas9 and gDonor content in the polymer nanoparticles. Gel electrophoresis was performed using a 4–20% Mini-PROTEAN TGX Gel (Bio-rad) in Tris/SDS buffer, with a loading dye containing 5% beta-mercaptoethanol. PageBlue solution (Thermo Fisher) staining was conducted and imaged with ChemiDoc MP using ImageLab software (Bio-rad). For particle size measurements, a dynamic light scattering study was conducted using a Zetasizer Nano ZS instrument (Malvern Instruments Ltd., Worcestershire, UK) and a folded capillary cell (DTS 1060, Malvern Instruments). The reported particle size was measured 5 min after particle mixing.

Lee K., Mackley V.A., Rao A., Chong A.T., Dewitt M.A., Corn J.E, & Murthy N. (2017). Synthetically modified guide RNA and donor DNA are a versatile platform for CRISPR-Cas9 engineering. eLife, 6, e25312.

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Zeta Potential and Size Determination

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A Zetasizer Nano ZS (Malvern Instruments, Worcestershire, UK) equipped with a 633nm laser was used to record electrophoretic measurements. A microfilter (0.22 µm) was utilised to filter each sample suspension prior to the measurements. The filtered samples were loaded onto a disposable capillary cell (DTS1060, Malvern). The cells loaded with samples were then flushed before reloading, and measurement s were made after the second loading. The obtained data were analysed using the software provided with the instrument employing the Smoluchowski equation to determine the particle size and zeta potential.

Mabrouk M., Beherei H.H., Tanaka Y, & Tanaka M. (2021). Investigating the Intermediate Water Feature of Hydrated Titanium Containing Bioactive Glass. International Journal of Molecular Sciences, 22(15), 8038.

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Characterization of Alginate-Calcium-pDNA Nanoparticles

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The mean diameter of AlgS-Ca²⁺-pDNA NPs was measured on a CGS-3 (ALV, Langen, Germany) instrument. Samples were diluted 1:50 in 10 mM HEPES solution (final concentrations of 2.5 or 5 mM Ca²⁺, 3 ng/μL pDNA, and 500 ng/mL AlgS); analyzed by scattered laser light (Helium-Neon [He-Ne] laser, 20 mW, 632.8 nm); and detected under an angle of 90° for the duration of 10 s, 20 times, at 25°C. Correlograms were calculated by ALV/LSE 5003 correlator and fitted with a version of the CONTIN program.
The size of AlgS-Ca²⁺-pDNA NPs was also determined by NTA. Samples were diluted 1:20 in 10 mM HEPES solution (final concentrations of 12.5 mM Ca²⁺, 7.5 ng/μL pDNA, and 1.25 μg/mL AlgS), and they were analyzed on a NanoSight NS300 instrument (Malvern Instruments, UK). Samples were analyzed under a 20× objective and 60-s video clips were taken. The software version NTA 2.3 was used for capture and analysis.
The Zeta potential of NPs (final concentrations of 2.5 or 5 mM Ca²⁺, 3 ng/μL pDNA, and 500 ng/mL AlgS) was measured on a Zetasizer Nano ZS (Malvern Instruments, UK), using electrophoretic cells (DTS 1060, produced by Malvern Instruments, UK). Zeta potentials were recorded 3 times with 10–100 measurements in each run (depending on SD).

Goldshtein M., Shamir S., Vinogradov E., Monsonego A, & Cohen S. (2019). Co-assembled Ca2+ Alginate-Sulfate Nanoparticles for Intracellular Plasmid DNA Delivery. Molecular Therapy. Nucleic Acids, 16, 378-390.

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CRISPR-Gold Synthesis and Characterization

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The synthetic intermediates in the synthesis of CRISPR-Gold, GNPs, GNP-DNA, GNP-DNA-donor DNA, GNP-Cas9 RNP and GNP-Cas9 RNP-Silicate were synthesized following the protocols described in S5 and characterized by UV-vis spectroscopy. The absorbance spectra of each sample were measured with a UV-vis spectrophotometer (NanoDrop 2000, Thermo Scientific). Dynamic light scattering (DLS) and zeta potential measurements were also made on each intermediate at 25 °C. Zeta potential measurements were made with a Zetasizer Nano ZS instrument (Malvern Instruments Ltd., Worcestershire, UK), and electrophoretic mobility was measured in a folded capillary cell (DTS 1060, Malvern Instruments). The zeta potential was calculated using the Smoluchowski equation. The size of the particles measured with DLS is reported in a number-based measurement mode. Each sample was prepared and incubated for a few minutes, to form particles, and then transferred to the capillary cell. An equilibration time ranging from and 2–5 minutes was needed to optimize the DLS measurements and collect accurate DLS data. Transmission electron microscopy (TEM) was conducted using a FEI Tecnai 12 microscope in the electron microscope lab at UC Berkeley. The samples were prepared on copper TEM grids (3.05 mm, 400 mesh).

Lee K., Conboy M., Park H.M., Jiang F., Kim H.J., Dewitt M.A., Mackley V.A., Chang K., Rao A., Skinner C., Shobha T., Mehdipour M., Liu H., Huang W.C., Lan F., Bray N.L., Li S., Corn J.E., Kataoka K., Doudna J.A., Conboy I, & Murthy N. (2017). Nanoparticle delivery of Cas9 ribonucleoprotein and donor DNA in vivo induces homology-directed DNA repair. Nature biomedical engineering, 1, 889-901.

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Nanoparticle Size and Zeta Potential Characterization

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Particle size distribution of freshly prepared BR Ext-loaded NGs and the empty ones were studied using dynamic light scattering (DLS) (Zetasizer Nano-ZSP, Malvern Instruments, Worcestershire, UK). All samples were obtained, analyzed, and interpreted conforming with previous works [19 (link),20 (link)]. Briefly, a multi-exponential function (CONTIN) was employed to establish the size–intensity of particle distribution and Mie theory was used to convert them to a size–volume distribution. This distribution gives the relative importance of each peak. Samples were contained in disposable polystyrene cuvettes (DTS0012, Malvern Instruments, Worcestershire, UK). EE was determined in the same device (Zetasizer Nano-ZSP, Malvern Instruments, Worcestershire, UK). Measurements were calculated by conversion of the electrophoretic mobility data to ζ-Pot using Henry’s equation [19 (link)]. To perform these determinations, samples were placed in disposable capillary cells (DTS1060, Malvern Instruments, Worcestershire, UK).

Silva Nieto R., Samaniego López C., Moretton M.A., Lizarraga L., Chiappetta D.A., Alaimo A, & Pérez O.E. (2023). Chitosan-Based Nanogels Designed for Betanin-Rich Beetroot Extract Transport: Physicochemical and Biological Aspects. Polymers, 15(19), 3875.

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Zeta Potential Measurement of nTiO2 and E. coli

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Zeta potential measures the total surface charge on particles and is independent of nanoparticle size and shape.⁴²Zeta potential changes if the chemistry of the exposure medium is altered. The zeta potential of nTiO₂ and E. coli was measured in Dryl’s buffer. Briefly, 1 ml of sample was injected into a capillary zeta cell (Folded Capillary Cell, DTS 1060, Malvern, UK). During injection of the sample, caution was taken so that bubbles did not form in the capillary cells and interfere with the analysis. Data were generated in the form of electrophoretic mobility, which was further converted to zeta potential by application of the Smoluchowki equation41 (link).

Gupta G.S., Kumar A., Shanker R, & Dhawan A. (2016). Assessment of agglomeration, co-sedimentation and trophic transfer of titanium dioxide nanoparticles in a laboratory-scale predator-prey model system. Scientific Reports, 6, 31422.

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Characterization of Nanoemulsions with Essential Oils

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The droplet size (DS), polydispersity index (PDI) and zeta potential (ZP) of oregano essential oil (nOEO), carvacrol (nCAR) and thymol nanoemulsions (nTHY) were determined in Dynamic Light Scattering (Zetasizer Nano^®, Model 590, Malvern Instruments, Malvern, UK). Samples were diluted 1:10 in ultrapure water, and values were determined by averaging three measurements at 25 °C. Data were reported as mean droplet diameter ± standard deviation (n = 3). Each sample was analyzed for size and PDI in a disposable polystyrene cell (DTS0012, Malvern Instruments, Malvern, UK). Disposable capillary cells (DTS 1060, Malvern Instruments, Malvern, UK) were used to measure the zeta potential. The pH of the nanoemulsions was measured with a pH meter (FiveEasy, Mettler Toledo, Jurong, Singapore).

da Silva B.D., do Rosário D.K., Neto L.T., Lelis C.A, & Conte-Junior C.A. (2023). Antioxidant, Antibacterial and Antibiofilm Activity of Nanoemulsion-Based Natural Compound Delivery Systems Compared with Non-Nanoemulsified Versions. Foods, 12(9), 1901.

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Nanoparticle Size and Zeta Potential Characterization

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Evaluating SPION Conjugation and Zeta Potential

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To evaluate the resulted size of conjugation, 5 µl of SPIONs or FGF2-SPIONs were diluted in 1 ml PBS and loaded in polystyrene cuvette. To evaluate the change in zeta potential, 5 µl of SPIONs or FGF2-SPIONs were diluted in 1 ml KCl 10 mM and loaded in folded capillary cells DTS1060 for zeta potential measurement (Malvern Panalytical). Examination of size or zeta potential of nanoparticles were performed with Zetasizer Nano ZS (Malvern Panalytical, Malvern, UK). Assays were performed with at least 2 mins equilibration and minimum 15 runs per measurement.

Mardhian D.F., Vrynas A., Storm G., Bansal R, & Prakash J. (2020). FGF2 engineered SPIONs attenuate tumor stroma and potentiate the effect of chemotherapy in 3D heterospheroidal model of pancreatic tumor. Nanotheranostics, 4(1), 26-39.

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Physical Characterization of Nanoparticle Emulsions

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Physical characteristics of all NEs were assessed by dynamic light scattering (DLS) using Malvern Instruments (Zetasizer Nano ZS). NEs were diluted at 1/2500 (v/v), and the average size and the polydispersity index were determined by three independent measurements performed at 25°C. To analyze the ζ-potential, NEs were diluted at 1/1500 (v/v), and measurements were performed using Zetasizer Nano ZS coupled with a folded capillary cell (DTS1060) from Malvern Instruments. Colloidal stability of loaded NEs was demonstrated for at least 3 months. The droplet’s mean diameter, index of polydispersity, and ζ-potential remained stable during the study. The macroscopic aspect showed no phase separation nor creaming during all study time.

Brouillard M., Barthélémy P., Dehay B., Crauste-Manciet S, & Desvergnes V. (2021). Nucleolipid Acid-Based Nanocarriers Restore Neuronal Lysosomal Acidification Defects. Frontiers in Chemistry, 9, 736554.

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