Trans blot turbo mini 0.2 m pvdf transfer packs set

DMU-214 was synthesized as described elsewhere [6 (link)]. The identity and purity of the compound were confirmed using NMR and LC–MS. Gefitinib was obtained from Sigma-Aldrich Co. (Darmstadt, Germany). The Cell Death Detection ELISA^PLUS kit was provided by Roche Diagnostics (Mannheim, Germany). Caspase-Glo^® -8, -9, -3/7 assay kits were purchased from Promega Co. (Madison, WI, USA). Super Signal West Pico PLUS Chemiluminescent Substrate was purchased from Thermo Scientific (Waltham, MA, USA). Trans-Blot Turbo Mini 0.2 µm PVDF Transfer Packs set, Quick Start Bradford Protein A, SsoAdvanced Universal Probes Supermix were provided by Bio-Rad (Hercules, CA, USA). All other materials were from Sigma-Aldrich Co. (St. Louis, MO, USA) unless otherwise stated.

All chemicals used were of analytical grade unless stated otherwise. DMU-212 was purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1’-rac-glycerol) (sodium salt) (POPG) were acquired from Avanti Polar Lipids (Alabaster, AL, USA). Chloroform and acetonitrile were purchased from Avantor Performance Materials Inc. (Radnor, PA, USA). The CellTiter-Glo® 3D Cell Viability Assay Kit was provided by Promega Co. (Madison, WI, USA). Estradiol and Progesterone ELISA kits were acquired from DRG Instruments GmbH (Marburg, Germany). Super Signal West Pico PLUS Chemiluminescent Substrate was purchased from Thermo Fisher Scientific (Waltham, Massachusetts, USA). The Trans-Blot Turbo Mini 0.2 µm PVDF Transfer Packs set and the Mini-PROTEAN® TGX™ Precast Gels were provided by Bio-Rad (Hercules, California, USA). All other materials were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA) unless otherwise stated.