Sodium octyl sulfate

Manufactured by Merck Group

Sourced in United States

Sodium octyl sulfate is a chemical compound that functions as a surfactant. It has a core role in various laboratory applications.

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Sodium sulfate (279 citations)

8 protocols using sodium octyl sulfate

Synthetic Polymeric Biomaterials Fabrication

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Sebacic acid (99.0%), malonic acid (99.0%), glutaric acid (99.0%), polycaprolactone triol (PCL triol, 300 Da), glycerol (99.0%), PEG (200, 1000, 2000 Da), N,N′-diisopropylcarbodiimide (DIC, 99.0%), 4-dimethylaminopyridine (DMAP, 99.0%), anhydrous N,N-dimethylformamide (DMF, 99.8%), anhydrous dimethyl sulfoxide (DMSO, 99.9%), anhydrous dichloromethane (DCM, 99.8%), deuterochloroform (99.96 atom% D), sodium octyl sulfate (SOS, 95.0%), dexamethasone (98.0%), fluorescein isothiocyanate isomer I (FITC, 90.0%), fluorescein sodium salt, hexamethylene diisocyanate (HMDI, 99.0%), dibutyltin dilaurate (Sn(II), 95.0%), and PBS (pH 7.4) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Cyanine5.5 carboxylic acid (Cy5.5, 95.0%) was purchased from Lumiprobe Corporation (Hallandale Beach, FL, USA). Tetrodotoxin (TTX) was obtained from Abcam (Cambridge, MA, USA). TTX ELISA kits were purchased from Reagen LLC (Moorestown, NJ, USA). Dulbecco’s minimum essential medium (DMEM), fetal bovine serum (FBS), and Penicillin Streptomycin was purchased from Thermo Fisher Scientific (Waltham, MA, USA).

Zhao C., Liu A., Santamaria C.M., Shomorony A., Ji T., Wei T., Gordon A., Elofsson H., Mehta M., Yang R, & Kohane D.S. (2019). Polymer-tetrodotoxin conjugates to induce prolonged duration local anesthesia with minimal toxicity. Nature Communications, 10, 2566.

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Neurochemical Compound Preparation

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Sodium chloride, potassium chloride, magnesium chloride, potassium phosphate monobasic, sodium phosphate, magnesium phosphate, sodium bicarbonate, calcium chloride, d-glucose, ascorbate, ethylenediaminetetraacetic acid (EDTA), sodium octylsulfate, acetonitrile, dopamine hydrochloride, and the dopamine D₂ receptor antagonist (−)-sulpiride were purchased from Sigma-Aldrich (St. Louis, MO). Ethanol (190 proof) was obtained from McCormick Distilling, Weston, MO. All chemicals were dissolved in the distilled water to desired concentrations.

Engleman E.A., Ingraham C.M., Rodd Z.A., Murphy J.M., McBride W.J, & Ding Z.M. (2020). The reinforcing effects of ethanol within the prelimbic cortex and ethanol drinking: Involvement of local dopamine D2 receptor-mediated neurotransmission. Drug and alcohol dependence, 214, 108165.

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Immunostaining of Astrocyte Markers

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Norepinephrine (NE) (Cat# A7257), rabbit liver glycogen (Cat# G8876), octanol (Cat# 297887), 18α-glycyrrhetinic acid (Cat# G8503), acetonitrile (Cat# 100030), quinine (Cat# 69311), dihydroxybenzylamine (Cat# 858781), sodium octyl sulfate (Cat# O4003), sulfuric acid (Cat# 339741) and a monoclonal anti-GFAP (RRID:AB_477010) antibody were obtained from Sigma Chemical Co. (St. Louis, MO, USA). Trypsin (Cat# 06369880103) and collagenase (Cat# 05349907103) were obtained from Roche CustomBiotech (Indianapolis, IN, USA). DME/F12 medium (Cat# 11320082) (1:1) and fetal bovine serum (FBS) (Cat# 16000044) were obtained from ThermoFisher (Grand Island, NY, USA). Phenol (Cat# 33517) was obtained from Sigma Chemical Co. (St. Louis, MO, USA). Goat anti-mouse IgG antibody conjugated to Cy5 (AlexaFluor 647) (RRID: AB_2535804) and donkey anti-rabbit IgG antibody conjugated to FITC (AlexaFluor 488) (RRID: AB_2535792) was obtained from ThermoFisher (Grand Island, NY, USA). Blocking peptides for Cx43 were synthetized in Genscript (Piscataway, NJ). Cx43 blocking peptide, Gap26, VCYDKSFPISHVR, and Scrambled peptide, PSFDSRHCIVKYV were used.

Eugenin E.A., Valdebenito S., Gorska A.M., Martínez A.D., Bitran M, & Sáez J.C. (2019). Gap junctions coordinate the propagation of glycogenolysis induced by norepinephrine in the pineal gland. Journal of neurochemistry, 151(5), 558-569.

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Quantitative Protein and Metabolite Analysis

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Commercial chemicals were of the highest purity available, common solvents were distilled before use and water was doubly distilled in a glass apparatus.
Paraformaldehyde (PFA), BSS, sucrose, fetal calf serum, Triton X-100, bovine serum albumin, trypan blue, perchloric acid, sodium acetate, sodium octyl sulfate, EDTA, DA, DOPAC and DAPI were from Sigma-Aldrich (St. Louis, MO, USA). Phosphate buffered saline (PBS) was from EuroClone. 4–20% Mini-PROTEAN TGX Precast Protein Gels, Turbo Polyvinylidene difluoride (PVDF) Mini-Midi membrane and DC protein assay kit were from Bio-Rad (Hercules, CA, USA). HPTLC plates were from Merck (Frankfurt, Germany). Optima cutting temperature (OCT) was from VWR (Radnor, PA, USA). Dako fluorescent mounting medium was from Dako (Glostrup, Denmark). Chemiluminescent kit for western blot was from Cyanagen (Bologna, Italy). Ultima gold was from PerkinElmer (Waltham, MA, USA).

Chiricozzi E., Mauri L., Lunghi G., Di Biase E., Fazzari M., Maggioni M., Valsecchi M., Prioni S., Loberto N., Pomè D.Y., Ciampa M.G., Fato P., Verlengia G., Cattaneo S., Assini R., Wu G., Alselehdar S., Ledeen R.W, & Sonnino S. (2019). Parkinson’s disease recovery by GM1 oligosaccharide treatment in the B4galnt1+/− mouse model. Scientific Reports, 9, 19330.

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Microcrystalline Cellulose-Based Surface Modification

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Microcrystalline cellulose (99%) with a primary particle diameter of 20 μm was purchased from Sigma. Cetyltrimethylammonium bromide (CTAB, 99%), dodecyltrimethylammonium bromide (DTAB, 98%), sodium dodecyl sulfate (SDS, 99%), sodium decyl sulfate (99%), and sodium octyl sulfate (99%) were all purchased from Sigma. Dodecane with a purity ≥99% was purchased from Aladdin and was columned two times through neutral alumina to remove possible polar impurities. Other chemicals were purchased from Sinopharm Chemical Reagent Co., which were all analytically pure. The ultrapure water used in all experiments with a resistance of 18.1 MΩ cm at 25°C was provided by Nantong University Analysis and Testing Center, China.

Zhu Y., Chen T., Cui Z., Dai H, & Cai L. (2020). Stimuli-Responsive Biomass Cellulose Particles Being Able to Reversibly Self-Assemble at Fluid Interface. Frontiers in Chemistry, 8, 712.

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Poly(propylene glycol) Functionalization

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Poly(propylene glycol) (PPG, Mn = 1000, 2000), potassium methoxide (KOCH₃, 95%), glycidol (96%), phosphate buffer saline (PBS, pH 7.4, 0.15M, 138mM NaCl, 2.7 mM KCl), chloroform-d (100%, 99.96 atom % D), fluorescein sodium salt, deuterium oxide (99.9 atom % D), dimethyl sulphoxide-[D6] (≥99.8%), fluorescein-5-isothiocyanate (FITC), Tissue-Tek® O.C.T. Compound were purchased from VWR International Ltd (Radnor, PA). Poly(propylene glycol) (PPG, Mn = 4000), and sodium octyl sulfate (≥95%), were purchased from Sigma-Aldrich Inc. (St. Louis, MO). Tetrodotoxin was purchased from Abcam (Cambridge, MA, USA). TTX ELISA kits were purchased from Reagen LLC (Moorestown, NJ, USA).

Li X., Li Q., Song S., Stevens A.O., Broemmel Z., He Y., Wesselmann U., Yaksh T, & Zhao C. (2022). Emulsion-induced polymersomes taming tetrodotoxin for prolonged duration local anesthesia. Advanced therapeutics, 6(1), 2200199.

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Neurotransmitter Quantification in Rat Brain

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KCl, CaCl₂, MgSO₄ and NaC₂H₃O₂ were purchased from Fisher Scientific (Fair Lawn, NJ, USA). NaCl, Na₂HPO₄·7H₂O, MgCl₂, d-glucose, sodium octyl-sulfate (SOS), ethylenediaminetetraacetic acid (EDTA), and acetonitrile were purchased from Sigma (St. Louis, MO, USA). KH₂PO₄ and NaHCO₃ were purchased from Acros Organics (NJ, USA). O-phthalaldehyde powder and diluents for glutamate derivatization were purchased from Pickering Laboratory, Inc, CA. The salts used in the aCSF and mobile phase were all HPLC grade. EtOH (Decon Laboratories, Inc, King of Prussia, PA, USA), the dopamine D_2/3 receptor antagonist (-)-sulpiride (St. Louis, MO, USA) and the mGlu_2/3 receptor antagonist LY341495 (Tocris Bioscience, Minneapolis, MN, USA) were dissolved in the aCSF to desired concentrations.

Ding Z.M., Ingraham C.M., Rodd Z.A, & McBride W.J. (2016). Alcohol drinking increases the dopamine-stimulating effects of ethanol and reduces D2 auto-receptor and group II metabotropic glutamate receptor function within the posterior ventral tegmental area of alcohol preferring (P) rats. Neuropharmacology, 109, 41-48.

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High-Performance Liquid Chromatography for DA Quantification

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The determination of DA level was performed by High performance liquid chromatography with electro-chemical detection (HPLC-EC). Chromatographic analysis was done using an Alltech 426 HPLC pump equipped with a Rheodyne injector, column length 15 cm, internal diameter 64.6 mm, Alltech Adsorbosphere C18 5U, electrochemical detector Antec CU-04-AZ and Varian Star Chromatographic Workstation. The mobile phase contained citric acid 0.5 M (99.5%, Sigma Aldrich), sodium acetate 1.0 M (NaAc, 99%, Sigma Aldrich), ethyl-enediaminetetraacetic acid 12.5 mM (EDTA, 98.5%, Sigma Aldrich), methanol 10% (MeOH, 99.93%, Sigma Aldrich) and sodium octyl sulfate 650 mg (pH 3.0, 95%, Sigma Aldrich); the flow rate was 1.3 mL min⁻¹.

Pinna A., Ricco' R., Migheli R., Rocchitta G., Serra P.A., Falcaro P., Malfatti L, & Innocenzi P. (2018). A MOF-based carrier for in situ dopamine delivery. RSC Advances, 8(45), 25664-25672.

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