At the three wetland sites, more than 788 days of observations were carried out for a total of ca. 11,820 h of direct observations. Each observation lasted 15 minutes in which every sighted bird was registered. These were made by experienced birdwatchers (two to three researchers in the field each day) using a Swarovski 20-60 telescope and Opticron Verano HD 10-42 binocular. Photographs were made with a Canon 60D camera (a database and Photo repository is available at http://lifecwr.com/index.php/pt/observacao/registos-de-observacao/registos-de-observacao-2 ). Whenever needed, several field-guides were used (e.g. Pereira 2010 , Mullarney et al. 2012 ), as well as websites on Azorean birds, namely AVES DOS AÇORES , Azores bird sightings and Birding Azores .
60d camera
Manufactured by Canon
Sourced in Japan
The Canon 60D is a digital single-lens reflex (DSLR) camera. It features a 18.0-megapixel APS-C-sized CMOS sensor, DIGIC 4 image processor, and a 3.0-inch vari-angle LCD screen. The camera is capable of recording full HD 1080p video at 30 frames per second.
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Lab products found in correlation
5 protocols using 60d camera
1
Comprehensive Avian Observation in Azorean Wetlands
2
Geometric Morphometric Analysis of Insect Wings
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The right forewing of every specimen was dissected and mounted in a water droplet between microscopic slides. In order to obtain repeatable pictures of wings that were as coplanar as possible, we removed the thick part at the base of the wings beforehand. Pictures were taken with a Visionary Digital imaging system, using Infinity lenses and a Canon 60D camera with a constant magnification.
Forewing size and shapes were assessed using 19 2D landmarks recorded with TPSDig2 software [30 ] (Fig 1 ). Landmarks of all specimens were superimposed once, using a generalized Procrustes analysis (GPA; [31 ]). This analysis extracts information of location, orientation and size from the raw landmark coordinates measured on the pictures in order to retain only the geometric shapes. Aligned landmark coordinates were then projected into the linear tangent space [32 ]. Shape variables were assessed as the scores of the 34 Principal Components (PC) with eigenvalues greater than zero from a Principal Component Analysis (PCA) on these tangent coordinates. In this multidimensional space, each point represented a potential wing shape and each vector depicted a shape change.
The forewing size was estimated by the log-transformed centroid size from the Procrustes analysis [33 ].
Forewing size and shapes were assessed using 19 2D landmarks recorded with TPSDig2 software [30 ] (
The forewing size was estimated by the log-transformed centroid size from the Procrustes analysis [33 ].
3
Evaluating Acne Treatments through Multimodal Assessment
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Photographs were taken with a digital camera (60D camera; Canon, Tokyo, Japan) and VISIA-CR (Canfield Scientific, Fairfield, NJ) before each session and 6 months after the final session. The patients filled out a questionnaire on-site or remotely, including the 5-point Global Aesthetic Improvement Scale (GAIS) [11 (link)] and Cardiff Acne Disability Index (CADI) [12 (link)]. Two independent dermatologists evaluated the Erythema Assessment Scale (EAS) [13 (link)] on the basis of the patients’ photographs. The persistence or clearing of lesions was evaluated on the basis of visual examination using standardised photography and quantitative analysis using the VISIA digital imaging system. Post-treatment responses including erythema, oedema and other side effects were recorded for each patient.
4
Quantitative Analysis of GUS Expression
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The expression of GUS reporter gene in Arabidopsis tissues was determined using a GUS staining kit (Solarbio, G3060) following the manufacturer’s instructions. The seedlings, leaves, flowers and siliques to be dyed were immersed in GUS dye solution and incubated overnight at 37 °C. The chlorophyll was removed with 75% ethanol until the background color disappeared completely. The results were documented by photography using a Canon 60d camera.
The material needed to determine Gus enzyme activity was frozen rapidly with liquid nitrogen, and then ground into powder by ball mill. The extraction buffer solution (50 mM NaH2PO4 (pH 7.0), 10 mM EDTA, 0.1% Triton X-100, 0.1 (w / v) sodium dodecyl sulfonate, 10 mM β-mercaptoethanol) were added to extract protein. After centrifugation at 4 °C, 12,000 r/min for 10 min, the supernatant was taken as protein extract. The protein concentration was determined by Bradford method. 4-MUG, the substrate of GUS reaction, was added and reacted at 37 °C for 30 min. Fluorescence measurement was carried out under the condition of 365 nm excitation light and 455 nm emission light. Three independent biological repeats were conducted. Finally, the GUS enzyme activity value was calculated according to the relative change of product in unit time.
The material needed to determine Gus enzyme activity was frozen rapidly with liquid nitrogen, and then ground into powder by ball mill. The extraction buffer solution (50 mM NaH2PO4 (pH 7.0), 10 mM EDTA, 0.1% Triton X-100, 0.1 (w / v) sodium dodecyl sulfonate, 10 mM β-mercaptoethanol) were added to extract protein. After centrifugation at 4 °C, 12,000 r/min for 10 min, the supernatant was taken as protein extract. The protein concentration was determined by Bradford method. 4-MUG, the substrate of GUS reaction, was added and reacted at 37 °C for 30 min. Fluorescence measurement was carried out under the condition of 365 nm excitation light and 455 nm emission light. Three independent biological repeats were conducted. Finally, the GUS enzyme activity value was calculated according to the relative change of product in unit time.
5
Sucrose Tolerance Assay for Arabidopsis
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For sugars tolerance, the seeds of WT and atsuc4 mutant lines were sown on MS solid media containing different concentrations of sucrose, glucose and mannitol after sterilizing the seeds (0%, 2%, 4% and 6%). Bright-eld images of different plants were captured at 6 d after sowing using Canon 60 D camera. Root length was measured by Image J software. For the phenotype comparision between WT and mutants, plants were grown in soil under LD condition. The number of rosette leaves was recorded at 25 d, and the plant height was measured at 50 d. Three biological replicate and more than 30 plants for each replicates were used for analysis. For seed germination rate, the WT and mutant seeds were sown on MS solid media containing different concentrations of sucrose (0%, 2%, 4% and 6%) and seed germination were calculated after 24 h, 48 h, 60 h and 72 h. Each treatment was performed with three biological replicates, at least comprising 100 seeds in each experiment.
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