Cell culture media, N,N,N′,N′-tetrakis (2-pyridylmethyl) ethylenediamine (TPEN), and fluorescent probes (Calcium Green-5N, Fluo-5N, FluoZin-1, FluoZin-2, FluoZin-3, fura-2, indo-1, Leadmium Green, mag-fura-2, Newport Green DCF, and Rhod-5N) were purchased from Thermo Fisher Scientific. fura-2FF was purchased from TEF Labs. Fetal bovine serum was purchased from Midwest Scientific. All other reagents were purchased from Sigma-Aldrich unless otherwise noted.
Newport green dcf
Manufactured by Thermo Fisher Scientific
Sourced in United States
Newport Green DCF is a fluorescent dye used in biological research. It is a cell-permeant, green-fluorescent indicator for nitric oxide (NO) that can be used to detect and measure NO levels in live cells.
Automatically generated - may contain errors
Lab products found in correlation
Fluozin 3, by Thermo Fisher Scientific (2 mentions)
Fura 2, by Thermo Fisher Scientific (2 mentions)
Calcium green 5n, by Thermo Fisher Scientific (2 mentions)
Indo 1, by Thermo Fisher Scientific (2 mentions)
Fluo 5n, by Thermo Fisher Scientific (2 mentions)
Fluozin 1, by Thermo Fisher Scientific (2 mentions)
Fluozin 2, by Thermo Fisher Scientific (2 mentions)
Leadmium green, by Thermo Fisher Scientific (2 mentions)
Rhod 5n, by Thermo Fisher Scientific (2 mentions)
Mag fura 2, by Thermo Fisher Scientific (2 mentions)
Facscalibur flow cytometer, by BD (1 mentions)
Bodipy, by Thermo Fisher Scientific (1 mentions)
Confocal laser scanning microscopy, by Leica (1 mentions)
4 protocols using newport green dcf
1
Cell Culture Media and Fluorescent Probes
2
Cell Culture Media and Fluorescent Probes
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Cell culture media, N,N,N′,N′-tetrakis (2-pyridylmethyl) ethylenediamine (TPEN), and fluorescent probes (Calcium Green-5N, Fluo-5N, FluoZin-1, FluoZin-2, FluoZin-3, fura-2, indo-1, Leadmium Green, mag-fura-2, Newport Green DCF, and Rhod-5N) were purchased from Thermo Fisher Scientific. fura-2FF was purchased from TEF Labs. Fetal bovine serum was purchased from Midwest Scientific. All other reagents were purchased from Sigma-Aldrich unless otherwise noted.
3
Quantitative Analysis of Cellular Zinc Uptake
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Analysis of Zn2+ uptake into cells was performed as previously described [29] (link). Cultured cells were incubated with 18 nM ZnCl2 in serum-free MEM that contained no detectable levels of Zn2+ for 60 min at 37°C in a humidified and equilibrated (5% v/v CO2) incubation. After washing with PBS, the cells were loaded with 5 µM zinc fluorescent sensor, Newport Green DCF (Invitrogen), for 30 min at 37°C in a humidified and equilibrated (5% v/v CO2) incubation. The cells were washed with PBS and the fluorescent intensities of the cells were analyzed using a FACSCalibur flow cytometer (BD Biosciences, CA, USA) at 488 nm laser excitation and a 515-545 nm emission filter.
4
Isolation and Analysis of Hepatocytes
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We isolated hepatocytes from yellow catfish liver tissues according to our recent publications [12 (link)]. After 48 h incubation, intracellular Zn and LD were detected based on the protocols of our previous studies [12 (link),31 (link)]. For detection of intracellular Zn2+ concentration, cells were incubated with 1 μM Newport Green DCF (Invitrogen, Carlsbad, CA, USA) for 30 min. For intracellular LD staining, cells were incubated with 5 mg/mL Bodipy (Invitrogen, Carlsbad, CA, USA) for 30 min. Fluorescence was imaged using laser scanning confocal microscopy (Leica, Wetzlar, Germany). We used the commercial assay kits (Nanjing Jian Cheng Bioengineering Institute, Nanjing, China) to analyze intracellular TG content. The mRNA levels of genes involved in lipid metabolism were examined by quantitative real-time PCR (Q-PCR). Primers are given in the Supplementary Table S6 .
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