AGEs were measured in serum samples by liquid chromatography–mass spectrometry (LC-MS) using internal stable heavy isotope substituted standards (Prevent AGE Healthcare Technology). Analysis was performed in duplicates in a blinded fashion on the serum filtrate following centrifugation through 10-K cutoff Amicon filter. This fraction contains free AGE as well as peptides of various sizes, and the analytical method measured the free products. An Agilent model 6490 Triple Quadrupole MS system with a 1290 Rapid Resolution LC system was used to detect analytes. All AGEs were separated and analyzed using Waters X-select HSS T3 column (2.5 mm, 2.1 3 150 mm) with a mobile phase gradient of methanol and water with 0.20% heptafluorobutyric acid. Total analysis time ran 19 min. Five dicarbonyl-derived AGEs were assessed: Ne-carboxymethyl lysine (CML), Ne-carboxyethyl lysine (CEL), glyoxalhydroimidazolone (G-H1), methylglyoxal hydroimidazolone (MG-H1), and 3-deoxyglucosone hydroimidazolone (3DG-H1).
1290 rapid resolution lc system
Manufactured by Agilent Technologies
Sourced in United States
The 1290 Rapid Resolution LC system is a high-performance liquid chromatography (HPLC) instrument designed for efficient and rapid separation and analysis of various chemical compounds. It features a modular design, allowing for customization to meet specific analytical requirements. The system is capable of delivering solvent flow rates up to 5 mL/min and operating at pressures up to 1200 bar, enabling fast and high-resolution separations.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using 1290 rapid resolution lc system
1
Quantification of AGEs in Serum Samples
2
Quantifying Advanced Glycation End-Products
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
AGEs were measured in a blinded fashion by liquid chromatography–mass spectrometry (LC-MS) using an Agilent model 6490 Triple Quadrupole MS with a 1290 Rapid Resolution LC system and stable heavy isotope substituted internal standards, as described elsewhere [25 (link)] (PreventAGE Healthcare Technology, Lebanon, NH, USA). Briefly, duplicate serum samples were centrifuged through a 10-K cutoff Amicon filter. The filtrate contains free AGEs and peptides of various sizes, as well as the analytical method measures the free products. AGEs were separated using a Waters X-select HSS T3 column (2.5 mm, 2.1 3 150 mm) with a mobile phase gradient of methanol and water with 0.20% heptafluorobutyric acid. Five dicarbonyl-derived AGEs were assessed: Ne-carboxymethyl lysine (CML), Ne-carboxyethyl lysine (CEL), glyoxalhydroimidazolone (G-H1), methylglyoxalhydroimidazolone (MG-H1), and 3-deoxyglucosone hydroimidazolone (3DG-H1) [25 (link)].
3
Quantifying AGEs and OxPs in Plasma
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
AGEs and OxPs were measured in plasma samples by liquid chromatography–mass spectrometry using internal stable heavy isotope–substituted standards (PreventAGE Health Care). Analysis was performed in a blinded fashion on the plasma filtrate after centrifugation through 10-kDa cutoff Amicon filters. This fraction contains free AGEs and OxPs as well as peptides of varied sizes, and the analytical method measured the free products. An Agilent model 6490 Triple Quadrupole LC/MS System with a 1290 Rapid Resolution LC System was used for analyte detection. All AGEs and OxPs were separated and analyzed in a single run using a single Waters X-select HSS T3 2.5 μm × 2.1 μm × 150 mm column with a mobile phase gradient of methanol/water with 0.20% heptafluorobutyric acid and a total analysis time of 19 min. The following seven biomarkers were measured: five dicarbonyl-derived AGE compounds—Nε-carboxymethyl lysine (CML), Nε-carboxyethyl lysine (CEL), glyoxal hydroimidazolone (G-H1), methylglyoxal hydroimidazolone (MG-H1), and 3-deoxyglucosone hydroimidazolone (3DG-H), and two oxidation products, methionine sulfoxide (MetSO) and 2-aminoaipic acid (2-AAA). Analyte interassay coefficients of variation were determined using a pooled plasma control measured in the 38 analytical runs performed during sample analysis. Interassay coefficients of variation varied from 3.6% (2-AAA) to 9.6% (G-H1).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!