PVA-117 (Mw = 145,000) and TA (Aladdin Reagent Co., Ltd., Shanghai, China). Hydroxyapatite (Macklin Reagent Co., Ltd., Shanghai, China). Mouse pre-osteoblasts (MC3T3-E1) cells (Fenghui Biotechnology Co., Changsha, China), Fetal bovine serum (FBS), alpha minimum essential medium (α-MEM), and penicillin/streptomycin (Gibco, Invitrogen, Carlsbad, CA, USA). CCK8 kit and Live/Dead assay kit (Beyotime Biological Co. Ltd., Shanghai, China), Deionized water (UPR-IV-10 T, China).
Hydroxyapatite
Manufactured by Maclin Biochemical Technology
Sourced in China
Hydroxyapatite is a naturally occurring mineral that is the primary constituent of human bone and tooth enamel. It is a crystalline calcium phosphate material that has a chemical formula of Ca10(PO4)6(OH)2. Hydroxyapatite is commonly used in various laboratory applications, such as chromatography and protein purification, due to its unique properties.
Automatically generated - may contain errors
4 protocols using hydroxyapatite
1
Osteoblast Differentiation Protocol
2
Fabrication of Multifunctional Drug-Eluting Scaffold
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Polyvinylpyrrolidone K30 (PVP K30, Mw ~ 40,000) was purchased from Sigma Aldrich (Shanghai, China). Polycaprolactone (PCL, Mw ~ 80,000), tranexamic acid (TA, 98%), hydroxyapatite (HAP, ≥97%), 2-(3-Phenoxyphenyl)propionic acid calcium salt dihydrate (fenoprofen, ≥98%), and FeCl3·6H2O (99%) were obtained from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). Solvents, including trifluoroethanol (TFE), dichloromethane (DCM), and ethyl alcohol (EtOH), were provided by Sinopharm Chemical Regent Co., Ltd. (Shanghai, China), and they are all analytical reagents. Water was double-distilled just before usage.
3
Biomimetic Hydroxyapatite Synthesis
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As we all know, the most important component of the tooth enamel is hydroxyapatite [24 (link)]. To mimic the binding process of PPi-Far-PMs with tooth enamel, we used a small intestinal submucosa as the bio-mineralization template to prepare plate-like, single-crystal hydroxyapatite, referred to henceforth as ‘biotechnological hydroxyapatite’. Meanwhile, we used commercially available hydroxyapatite (Macklin, Shanghai, China) as the control.
4
Biomimetic Hydroxyapatite Synthesis
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As we all know, the most important component of the tooth enamel is hydroxyapatite [24] . To mimic the binding process of PPi-Far-PMs with tooth enamel, we used a small intestinal submucosa as the biomineralization template to prepare plate-like, single-crystal hydroxyapatite, referred to henceforth as 'biotechnological hydroxyapatite'. Meanwhile, we used commercially available hydroxyapatite (Macklin, Shanghai, China) as the control.
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