Clean up mini kit

Chromosomal DNA was extracted using the Genomic Mini Kit (A&A Biotechnology, Gdynia, Poland), according to the manufacturer’s instructions. The amplification of the bacterial 16S rRNA gene was performed with the universal primer pair 27f and 1492r (25 ). In the identification of yeast strains, fragments of approximately 500–600 bp, corresponding in size to the ITS1, ITS2 region and the 5.8 ribosomal RNA gene, were amplified with the primer set ITS1 and ITS4 (44 ).
All PCR reactions were conducted using the MJ Mini Gradient Thermal Cycler (Bio-Rad, Hercules, CA, USA) in a mixture containing 40 pmol of each primer, 1.5 U of RedTaq ReadyMix DNA polymerase (Sigma-Aldrich, St. Louis, MO, USA), and 20 ng of template DNA diluted to 50 μL with DNase-free water. PCR products were detected by electrophoresis in 1% agarose gel (w/v) in 0.5×TBE buffer (Sigma-Aldrich, St. Louis, MO, USA) and purified using the Clean-Up Mini Kit (A&A Biotechnology, Gdynia, Poland) following the manufacturer’s protocol.

2-2-Diphenyl-1-picrylhydrazyl (DPPH); Folin-Ciocalteu’s reagent (2 N); (±)-6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox); (+)-catechin; and 2,4,6-tri(2-pyridyl)-s-triazine (TPTZ), linoleic acid, Tween 40, iron (II) sulfate heptahydrate, iron (III) chloride hexahydrate, β-carotene, RedTaq ReadyMix DNA polymerase, agarose gel electrophoresis and TBE buffer were purchased from Sigma-Aldrich (St. Louis, Missouri, USA). Plate count agar (PCA), DG18 medium, and bullion agar were purchased from Merck (Darmstadt, Germany). The oxidase test kit was obtained from Merck (Darmstadt, Germany). API Tests were purchased from BioMérieux (France). Genomic Mini kit and Clean Up Mini Kit were purchased from A&A Biotechnology (Gdynia, Poland). MJ Mini Gradient Thermal Cycler was obtained from Bio-Rad, (Hercules, CA, USA). BigDye Terminator Ready Reaction Cycle Sequencing kit was purchased from Applied Biosystems (Foster City, CA, USA). Unless indicated otherwise, all chemicals were purchased from Avantor Performance Materials Poland S.A. (Gliwice, Poland).