FreeStyle293F cells (ThermoFisher Scientific) and HEK293-S cells (ThermoFisher Scientific) were cultured in FreeStyle293 expression media (Life Technologies), cultured at 37°C with 8% CO2 while shaking at 130 rpm. HEK293-T cells (ATCC) and Vero E6 cells (ATCC) were cultured at 37°C in the presence of 5% CO2 in DMEM supplemented with 10% heat-inactivated FBS, 1% penicillin, 1% streptomycin, 2 mM l-glutamine, non-essential amino acids (Invitrogen) and 1 mM sodium pyruvate. Huh7.5 cells (provided by Dr. Deborah R. Taylor) were cultured at 37°C with 8% CO2 in flasks with DMEM + 10% FBS. ExpiCHO-S cells (GIBCO) were cultured at 37°C with 8% CO2 while shaking at 130 rpm in ExpiCHO expression media (GIBCO). Cells lines were not tested for mycoplasma contamination nor authenticated.
Expicho expression media
Manufactured by Thermo Fisher Scientific
ExpiCHO expression media is a cell culture medium designed for the transient expression of recombinant proteins in Chinese Hamster Ovary (CHO) cells. It provides the essential nutrients and growth factors required to support the rapid growth and high-level protein production of CHO cells in suspension culture.
Automatically generated - may contain errors
Lab products found in correlation
Expicho s cells, by Thermo Fisher Scientific (2 mentions)
L glutamine, by Thermo Fisher Scientific (1 mentions)
Hek293t cell, by American Type Culture Collection (1 mentions)
Vero e6 cell, by American Type Culture Collection (1 mentions)
Non essential amino acids (neaa), by Thermo Fisher Scientific (1 mentions)
Freestyle 293 f cells, by Thermo Fisher Scientific (1 mentions)
Freestyle 293 expression media, by Thermo Fisher Scientific (1 mentions)
Ecori, by Takara Bio (1 mentions)
Hitrap, by Cytiva (1 mentions)
Mabselect, by Cytiva (1 mentions)
2 protocols using expicho expression media
1
Cell Culture Conditions for Multiple Cell Lines
2
Production of Evinacumab-Fc Fusion Protein
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The encoding sequence of C44 fused to human IgG1-Fc (with the hinge region and had mutated the first Cys into Ser) was synthesized and inserted into pTT5 vector. The DNA sequences encoding the variable regions of evinacumab were cloned and digested into corresponding regions of human IgG1 on pTT5 vector by EcoRI and NheI (Takara, Biotechnology, Otsu, Japan). The C44-Fc and evinacumab expression plasmids were transfected into ExpiCHO-S cells (Gibco, Thermo Scientific Ltd.) by CHO Transfection Kit and cultured in ExpiCHO expression media (Gibco, Thermo Scientific Ltd.) according to its Max Titer protocol. The culture supernatants were collected 12 days after transfection and purified by HiTrap MabSelect Prism A column (GE Healthcare).
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