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Pierce rna pull down kit

Manufactured by Thermo Fisher Scientific
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The Pierce RNA pull-down Kit is a tool designed to facilitate the study of protein-RNA interactions. It provides a method for the purification and identification of RNA-binding proteins associated with specific RNA sequences.

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Lab products found in correlation

T7 rna polymerase, by Promega (1 mentions)

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Pierce Magnetic RNA-Protein Pull-Down Kit (586 citations) Pierce Magnetic RNA Pull-Down Kit (12 citations) Thermo Scientific Pierce Magnetic RNA-Protein Pull-Down Kit (3 citations)

2 protocols using pierce rna pull down kit

1

RNA-Protein Interaction Profiling

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RNA pull-down assay was conducted using Pierce RNA pull-down Kit (Thermo Fisher Scientific). In brief, oligo probe (negative control) or circPOFUT1/miR-635-5p probe was conjugated with streptavidin beads and incubated with cell lysates. RNA was extracted using Trizol reagent, and subjected to qRT-PCR analysis.
Long F., Tian B., Li L., Ma M., Chen Z., Tan G., Yin N., Zhong C., Yu B., Guo Y., Chen M, & Hu G. (2023). CircPOFUT1 fosters colorectal cancer metastasis and chemoresistance via decoying miR-653-5p/E2F7/WDR66 axis and stabilizing BMI1. iScience, 27(1), 108729.
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2

RNA-Protein Interaction Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
RNA pull-down assay was conducted as per the instructions of Pierce™ RNA Pull-Down Kit (20164, Thermo Scientific). DUSP1 and DUSP1 antisense (served as negative control) were ligated into pcNDA 3.1 vector and subsequently transcribed in vitro using T7 RNA polymerase (p2075, Promega). These RNAs were labeled by biotin using Pierce™ RNA 3′ End Desthiobiotinylation, and the mixture was treated with prewashed Pierce™ Streptavidin Magnetic Beads. Then, these beads were incubated with cell extracts at 4°C overnight. After washing non-specific binding, the hybridized RNAs were extracted using TRIzol and detected by RT-qPCR.
Li Y., Shao J., Song J., Yu S., Wang J, & Sun K. (2021). MiR-34a-3p suppresses pulmonary vascular proliferation in acute pulmonary embolism rat by targeting DUSP1. Bioscience Reports, 42(1), BSR20210116.
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