Human ovarian cancer SKOV3 cells (CL-0215), Human breast cancer MCF−7 cells (CL-0149), Human brain glioma U87 cells (CL-0238), Mouse melanoma B16F10 cells (CL-0319), Human hepatic stellate LX2 cells (CL-0560), Human hepatocellular carcinomas HepG2 cells (CL-0103) and Human breast cancer MDA-MB-231 cells (CL-0150) were kindly provided by Procell Life Science & Technology Co., Ltd. Authentication of all cells was conducted via short tandem repeat (STR) profiling in Procell Life Science & Technology Co., Ltd.
Mda mb 231 cells
Manufactured by Procell
Sourced in China
MDA-MB-231 cells are a type of human breast cancer cell line. They are commonly used in research for studying various aspects of breast cancer, including cell growth, signaling pathways, and response to treatments.
Automatically generated - may contain errors
Lab products found in correlation
Mcf 7 cells, by Procell (1 mentions)
B16f10 cells, by Procell (1 mentions)
Skov3 cells, by Procell (1 mentions)
U87 cells, by Procell (1 mentions)
Thp 1, by Procell (1 mentions)
Hela cells, by Procell (1 mentions)
Milli q device, by Merck Group (1 mentions)
Thioacetic acid, by Merck Group (1 mentions)
Concanavalin a (cona), by Merck Group (1 mentions)
Cell counting kit 8 cck 8, by Dojindo Laboratories (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Thp 1 cell, by Procell (1 mentions)
Mda mb 468 cells, by American Type Culture Collection (1 mentions)
7 protocols using mda mb 231 cells
1
Cell Line Provenance Validation
2
Cell Culture Protocol for MDA-MB-231 and THP-1
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MDA-MB-231 cells and the human monocytic cell line THP-1 were purchased from Procell Life Science & Technology Co., Ltd. Cells were cultured in DMEM containing 10% fetal bovine serum and 1% penicillin‒streptomycin at 37°C and 5% CO2 . Cells were digested using ztrypsin upon reaching 80% confluence, and the cells in the logarithmic growth phase were used for subsequent experiments.
3
Culturing Common Cancer Cell Lines
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The HeLa, A549, MCF-7, A2780, SW620 cell lines, and the liver cancer cell line, HepG2 were purchased from the China Center for Typical Culture Collection. The MDA-MB-231 cells were purchased from Procell Life Science and Technology Co., Ltd.. The HeLa cells were cultured in DMEM supplemented with 10% (v/v) NBS, 100 µg/ml streptomycin, and 100 IU/ml penicillin at 37°C in a humidified incubator with 5% CO2. The A549 cells were maintained in RPMI-1640, supplemented with 10% (v/v) FBS, while the MCF-7, HepG2, A2780, SW620, MDA-MB-231 cells were maintained in DMEM supplemented with 10% (v/v) FBS.
4
Glycan-Decorated Gold Nanoparticles
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The materials and reagents in this work were in analytical-reagent grade and used directly without further purification. Chloroauric acid hydrated (HAuCl4·4H2O) was purchased from Chemical Reagent Co., Ltd. of Shanghai (Shanghai, China). L-Glutathione reduced (GSH), methoxylamine hydrochloride, sodium cyanoborohydride, sodium acetate an-hydrous, dichloromethane, ethanol, hydroxylamine hydro-chloride (NH2OH·HCl), D-mannose (Man), D-glucose (Glu), D-lactose monohydrate (Lac), monosialoganglioside (GM3), and phenol were obtained from Yinuokai Technology Co., Ltd. (Beijing, China). 4-Maleimidobutyric acid N-hydroxysuccinimide ester (GMBS), acrolein, L-fucose (Fuc), and 4-aminophenyl α-D-mannopyranoside (NH2-Man) were provided by Aladdin Industrial Corporation (Shanghai, China). Thioacetic acid and concanavalin A (Con A) were obtained from Sigma Aldrich (St. Louis, MO, USA). The cell counting kit-8 (CCK-8) was purchased from Dojindo Laboratories (Kyushu, Japan). The human breast cancer MDA-MB-231 cells and Swiss albino 3T3 cells were purchased from Procell Life Technology Co., Ltd. (Wuhan, China). The ultrapure water (18.2 MΩ) used in this work was generated by a Milli-Q device (Millipore, MA, USA).
5
Overexpression of IGF2BP2 in Breast Cancer
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MDA-MB-231 cells were purchased from Procell Life Science & Technology Co. Ltd. They were cultured in Dulbecco’s Modified Eagle Medium (DMEM) medium, containing 10% fetal bovine serum and antibiotics, at 37℃, with 5% CO2. For transfection, the cells were divided into three groups: the control group, the IGF2BP2 overexpression (Ov) group, and the IGF2BP2 IGF2BP2 Ov negative control (NC) group. The cells were plated in 6-well plates until cell confluence reached 90%, after which cell transfection was performed using Lipofectamine 2000. At 48 h after transfection, the cells were collected for analysis.
6
Cell Culture and Differentiation Protocol
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MDA-MB-231 cells and THP-1 cells (the human monocytic cell line) were obtained from Procell Life Science & Technology Co., Ltd. (Wuhan, China), and MDA-MB-468 cells from the American Type Culture Collection were grown in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum and 1% penicillin‒streptomycin (Invitrogen) at 37°C. Upon reaching 80–90% confluence, cells were passaged at a ratio of 1:5–1:10, and the following assays were conducted with cells in the logarithmic growth phase. The differentiation of THP-1 cells was induced by incubation with 50 ng/mL phorbol 12-myristate 13-acetate (PMA) for 48 h.
7
Cell Culture Protocols for Cancer and Normal Cells
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MDA-MB-231 cells were obtained from Procell Life Science & Technology Co., Ltd. They were cultivated in DMEM medium enriched with 10% FBS and 1% penicillin/strepto mycin antibiotic. MCF-10A cells were also used in this study. They were cultured in DMEM/F 12 supplemented with 20ng/ml EGF, Hydrocortisone, Insulin, NEAA, 5% HS, and 1% penicillin/streptomycin Solution. The manufacturer's recommended protocols were followed for maintaining the cells. All cell cultures were maintained in a humid incubator at 37°C with 5% CO2.
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