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Human cgrp

Manufactured by Merck Group
Sourced in United States

Human CGRP is a laboratory reagent used for research purposes. It is a protein that functions as a potent vasodilator, playing a role in the regulation of vascular tone and blood flow. This product is intended for use in scientific research and experiments, not for diagnostic or therapeutic use.

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2 protocols using human cgrp

1

CGRP Treatment of THP-1 Cells

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Human CGRP (Sigma Aldrich, Saint Louis, Missouri, USA) was dissolved in dimethyl sulfoxide (DMSO; Sigma Aldrich, Saint Louis, Missouri, USA), further diluted in Dulbecco’s phosphate-buffered saline (DPBS; Sigma-Aldrich, Saint Louis, Missouri, USA) and stored at −20 °C until use. During the experiments, THP-1 cells were treated with a commonly used final concentration of 10−8 M CGRP often shown to exert maximal effects in cell culture [10 (link), 12 (link)–16 (link)] while equal amounts of DPBS were added to the corresponding controls.
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2

Investigating Vascular Inflammation Pathways

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Human CGRP, Human CGRP8–37, Ang II, N‐acetyl‐L cysteine (NAC), apocynin, PP2, H‐89 and dibutyl‐cAMP were purchased from Sigma. Niclosamide was purchased from Selleck Chemicals; anti‐phospho‐Src (Tyr416), anti‐phospho‐STAT3 (Tyr705), Src and STAT3 antibodies, from Cell Signaling Technology Inc; antibodies for p47phox and GAPDH, and all secondary antibodies, from Santa Cruz Biotechnology; antibodies of RAMP1, CRLR and ATP1a1, from Santa Cruz Biotechnology; RCP antibodies, from NOVUS Institute of Biotechnology; Alzet mini‐osmotic pumps (Alzet model 1004), from DURECT Corp; and the Src (B0107) and STAT3 (F0806) ORF cDNA clones, from GeneCopoeia. Other chemicals and reagents were of analytical grade.
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