Human CGRP (Sigma Aldrich, Saint Louis, Missouri, USA) was dissolved in dimethyl sulfoxide (DMSO; Sigma Aldrich, Saint Louis, Missouri, USA), further diluted in Dulbecco’s phosphate-buffered saline (DPBS; Sigma-Aldrich, Saint Louis, Missouri, USA) and stored at −20 °C until use. During the experiments, THP-1 cells were treated with a commonly used final concentration of 10−8 M CGRP often shown to exert maximal effects in cell culture [10 (link), 12 (link)–16 (link)] while equal amounts of DPBS were added to the corresponding controls.
Human cgrp
Manufactured by Merck Group
Sourced in United States
Human CGRP is a laboratory reagent used for research purposes. It is a protein that functions as a potent vasodilator, playing a role in the regulation of vascular tone and blood flow. This product is intended for use in scientific research and experiments, not for diagnostic or therapeutic use.
Automatically generated - may contain errors
Lab products found in correlation
Dulbecco s phosphate buffered saline dpbs, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Niclosamide, by Selleck Chemicals (1 mentions)
Apocynin, by Merck Group (1 mentions)
Antibodies for p47phox and gapdh, by Santa Cruz Biotechnology (1 mentions)
Dibutyl camp, by Merck Group (1 mentions)
Ang 2, by Merck Group (1 mentions)
N acetyl l cysteine, by Merck Group (1 mentions)
Alzet mini osmotic pumps alzet model 1004, by Durect (1 mentions)
2 protocols using human cgrp
1
CGRP Treatment of THP-1 Cells
2
Investigating Vascular Inflammation Pathways
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Human CGRP, Human CGRP8–37, Ang II, N‐acetyl‐L cysteine (NAC), apocynin, PP2, H‐89 and dibutyl‐cAMP were purchased from Sigma. Niclosamide was purchased from Selleck Chemicals; anti‐phospho‐Src (Tyr416), anti‐phospho‐STAT3 (Tyr705), Src and STAT3 antibodies, from Cell Signaling Technology Inc; antibodies for p47phox and GAPDH, and all secondary antibodies, from Santa Cruz Biotechnology; antibodies of RAMP1, CRLR and ATP1a1, from Santa Cruz Biotechnology; RCP antibodies, from NOVUS Institute of Biotechnology; Alzet mini‐osmotic pumps (Alzet model 1004), from DURECT Corp; and the Src (B0107) and STAT3 (F0806) ORF cDNA clones, from GeneCopoeia. Other chemicals and reagents were of analytical grade.
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