Sensitive and specific immunoassays

Manufactured by R&D Systems

Sensitive and specific immunoassays are analytical tools used to detect and quantify target analytes in a sample. These assays utilize the highly specific binding interactions between an antibody and its corresponding antigen to provide accurate and precise measurements. They are designed to offer enhanced sensitivity and selectivity, enabling the detection of low-abundance analytes in complex matrices.

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Specific immunoassays

2 protocols using sensitive and specific immunoassays

Plasma sST2 Quantification Protocol

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Blood samples were collected into tubes containing EDTA. The samples were centrifuged for 10 min at 4°C and stored at −70°C. Laboratory personnel were blinded to the clinical diagnoses. Maternal plasma concentrations of sST2 were determined using sensitive and specific immunoassays (R&D Systems, Minneapolis, MN). All immunoassays utilized a sandwich enzyme-based technique and had been validated for plasma determinations of the analytes. The inter- and intra-assay coefficients of variation were 4.6% and 3.9%, respectively. The sensitivity of the assays was 17.5 pg/mL.

Romero R., Chaemsaithong P., Tarca A.L., Korzeniewski S.J., Maymon E., Pacora P., Panaitescu B., Chaiyasit N., Dong Z., Erez O., Hassan S.S, & Chaiworapongsa T. (2017). Maternal plasma soluble ST2 concentrations are elevated prior to the development of early and late onset preeclampsia – a longitudinal study. The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians, 31(4), 418-432.

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Maternal Plasma Biomarker Quantification

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Maternal plasma was collected into tubes containing EDTA. Samples were centrifuged and stored at −70°C. Sensitive and specific immunoassays (R&D Systems, Minneapolis, MN) utilizing a quantitative sandwich enzyme technique were used to determine maternal plasma concentrations of PlGF, sEng, and sVEGFR-1. The inter- and intra-assay coefficients of variation were: PlGF, 6.02 and 4.8%; sEng, 2.3 and 4.6%; sVEGFR-1, 1.4 and 3.9%, respectively. The lower limits of the detection were: PlGF, 9.52 pg/mL; sEng, 0.08 ng/mL; and sVEGFR-1, 16.97 pg/mL.

Chaiworapongsa T., Romero R., Whitten A.E., Korzeniewski S.J., Chaemsaithong P., Hernandez-Andrade E., Yeo L, & Hassan S.S. (2016). The use of angiogenic biomarkers in maternal blood to identify which SGA fetuses will require a preterm delivery and mothers who will develop preeclampsia. The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians, 29(8), 1214-1228.

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