Pharmascan mri system

Data were acquired on a 7T Bruker PharmaScan MRI system and operated via ParaVision 5.1 software (Bruker BioSpin MRI GmbH, Ettlingen, Germany). Signal was transmitted with a volume resonator (inner diameter 72 mm) and received with a rat brain 4-channel quadrature surface coil (both from Bruker BioSpin). fMRI data were collected using a spin-echo EPI sequence with the following parameters: repetition time 2 s, echo time 45 ms, field of view 25 × 25 mm², matrix size 64 × 64 (in-plane resolution of 0.39 × 0.39 mm²), 9 contiguous coronal slices with 1.5 mm thickness, and acquisition time 600 s (300 volumes). The fMRI slices were placed from the posterior end of the olfactory bulb to the anterior end of the cerebellum (see fMRI coverage in Figure 5—figure supplement 5).

Animal MRI was performed on a 7T/16 cm Bruker PharmaScan MRI system. Mice were anesthetized with isoflurane in 100% oxygen and maintained at 40-80 breaths per minute. Animals were scanned by MRI prior to or after dosing with EuS (17 mg/kg, tail vein injection). T2-relaxtion maps were generated using a CPMG phase-cycled multi-echo multi-slice sequence. Data were acquired with 3000 ms repetition time, 50 echoes (echo times TEn = n × 10 ms; n = 1), 256 × 128 acquisition matrix, 25 × 25 mm FOV, slice thickness = 0.5 mm, 2 averages, for a total scan time of 13 min. T2 signal in liver, spleen, and kidneys was quantified by densitometry of ROI pixilation in ImageJ v1.51m9 (National Institutes of Health, USA) 21 (link) and converted to ΔR2 as 1000/(T2_{post-treatment} - T2_{pre-treatment}).