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Cat 8884

Manufactured by Cell Signaling Technology
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The Cat 8884 is a laboratory equipment product offered by Cell Signaling Technology. Its core function is to provide a reliable and efficient tool for researchers in the field of cell biology and biochemistry. The detailed specifications and intended use of this product are not available in this unbiased and factual description.

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Lab products found in correlation

Western lightning plus, by PerkinElmer (2 mentions) Ibright cl1000, by Thermo Fisher Scientific (2 mentions) Non fat dry milk, by Bio-Rad (2 mentions)

2 protocols using cat 8884

1

Western Blot Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
After samples were run on 4–20% gels, they were transferred to nitrocellulose membranes. Membranes were blocked in 5% (w/v) nonfat dry milk (Bio-Rad, Hercules, CA, USA) in TRIS-buffered saline (TBS) with 0.1% (v/v) Tween-20 (TBST) and then incubated with primary antibodies in 1% (w/v) nonfat dry milk. After washing with TBST, membranes were incubated with horseradish peroxidase-linked secondary antibodies. Membranes were washed with TBST and then TBS before incubation with Western Lightning Plus (PerkinElmer, Waltham, MA, USA) enhanced chemiluminescence reagent, followed by CCD imaging (iBright CL1000, ThermoFisher Scientific). The primary antibody against GAPDH (Cell Signaling Technology Cat 8884, RRID:AB11129865) was conjugated to horseradish peroxidase, so there was not a need for incubation with secondary antibodies.
Eccardt A.M., Pelzel R.J., Bell T.P, & Fisher J.S. (2022). Stimulation of Akt Phosphorylation and Glucose Transport by Metalloporphyrins with Peroxynitrite Decomposition Catalytic Activity. Catalysts (Basel, Switzerland), 12(8), 849.
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2

Western Blot Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
After samples were run on 4–20% gels, they were transferred to nitrocellulose membranes. Membranes were blocked in 5% (w/v) nonfat dry milk (Bio-Rad, Hercules, CA, USA) in TRIS-buffered saline (TBS) with 0.1% (v/v) Tween-20 (TBST) and then incubated with primary antibodies in 1% (w/v) nonfat dry milk. After washing with TBST, membranes were incubated with horseradish peroxidase-linked secondary antibodies. Membranes were washed with TBST and then TBS before incubation with Western Lightning Plus (PerkinElmer, Waltham, MA, USA) enhanced chemiluminescence reagent, followed by CCD imaging (iBright CL1000, ThermoFisher Scientific). The primary antibody against GAPDH (Cell Signaling Technology Cat 8884, RRID:AB11129865) was conjugated to horseradish peroxidase, so there was not a need for incubation with secondary antibodies.
Nishio H, & Dugaiczyk A. (1996). Complete structure of the human alpha-albumin gene, a new member of the serum albumin multigene family.. Proceedings of the National Academy of Sciences of the United States of America, 93(15), 7557-7561.
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