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Il 1β emc001b

Manufactured by Neobioscience
Sourced in China

IL-1β (EMC001b) is a recombinant protein produced in E. coli. It is a member of the interleukin-1 cytokine family and plays a crucial role in the regulation of immune and inflammatory responses.

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2 protocols using il 1β emc001b

1

Cytokine and Oxidative Stress Evaluation

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Cytokine levels in the vitreous humor or conditioned media were detected using ELISA kits following the manufacturer’s instructions, including IL-1β (EMC001b; NeoBioScience, Shenzhen, China) and IL-18 (EHC127.48; NeoBioScience). Vitreous humor malondialdehyde (MDA) activity was measured using the thiobarbituric acid method (S0131S; Beyotime Biotechnology, Shanghai, China), and SOD activity was measured using the xanthine oxidase method (S0101S; Beyotime Biotechnology).
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2

Cytokine Quantification in BMDM Cells

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Enzyme‐linked immunosorbent assay kits were used to measure the concentration of IL‐1β, IL‐6 and TNF‐α in supernatant of BMDM cells following the instruction of kits (IL‐1β [EMC001B], IL‐6 [EMC004] and TNF‐α [EMC102A], Neobioscience). The concentration of each cytokine was defined by the absorbance measured with a microplate reader at 450 nm.
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