Gfp 470 510

mKate2 expressing U2OS cells were seeded either at 200,000 cells per well of 6-well plates, or at 2,500 cells on 96-well plates and adhered O/N. On day 0, drugs were added to wells in addition to 50 nM SYTOX. Images were captured using an EVOS FL Auto microscope with a 10X objective using GFP (470/510) and Texas Red (585/624) light cubes (Life Technologies). 25 images were collected per well for experiments on 6-well plates, and 4 images per well for experiments on 96-well plates, with 2 wells per treatment condition. Live cell and dead cell counting was performed using the incucyte software. The average number of total cells counted per image was 284 (range 108 – 812 cells per image). Lethal fraction was computed as in Forcina et al.^{21 (link)}