Apc conjugated mouse anti cd44

Manufactured by BD

Sourced in United States

The APC)-conjugated mouse anti-CD44 is a monoclonal antibody that binds to the CD44 cell surface glycoprotein. CD44 is involved in cell-cell and cell-matrix interactions, cell migration, and signal transduction.

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Lab products found in correlation

Pe conjugated mouse anti cd24, by BD (1 mentions) Facsymphony a5 laser analyzer, by BD (1 mentions) Alexa 488 conjugated goat anti rabbit, by Thermo Fisher Scientific (1 mentions) Pe conjugated mouse anti cd133, by Miltenyi Biotec (1 mentions) Mouse anti o glcnac rl2, by Thermo Fisher Scientific (1 mentions) Rabbit anti β tubulin, by Cell Signaling Technology (1 mentions) Rabbit anti cd44, by Abcam (1 mentions) Rabbit anti cd133, by Abcam (1 mentions)

Close spelling variants of this product

APC-conjugated rat anti-mouse CD44 APC-conjugated anti-CD44 APC-conjugated CD44 Anti-CD44-APC Mouse anti-CD44 CD44-APC Anti-CD44 Mouse anti

2 protocols using apc conjugated mouse anti cd44

Immunophenotyping of Cell Surface Markers

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Approximately 1 × 10⁶ cells were incubated for 15 min with allophycocyanin (APC)-conjugated mouse anti-CD44 (BD Biosciences, 560890) and phycoerythrin ( PE)-conjugated mouse anti-CD24 (BD Biosciences, 560991) according to instructions provided by the manufacturer. Cells were analyzed using a flow cytometer instrument (BD FACSymphony A5-Laser Analyzer) equipped with FACSDiva software.

Coelho D.R., Palma F.R., Paviani V., He C., Danes J.M., Huang Y., Calado J.C., Hart P.C., Furdui C.M., Poole L.B., Schipma M.J, & Bonini M.G. (2022). Nuclear-localized, iron-bound superoxide dismutase-2 antagonizes epithelial lineage programs to promote stemness of breast cancer cells via a histone demethylase activity. Proceedings of the National Academy of Sciences of the United States of America, 119(29), e2110348119.

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Investigating Cancer Stem Cell Markers

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The following antibodies were used in the experiments: allophycocyanin (APC)-conjugated mouse anti-CD44 from BD Biosciences, phycoerythrin (PE)-conjugated mouse anti-CD133 from Miltenyi Biotec, rabbit anti-CD44, rabbit anti-CD133, and mouse anti-OGT from Abcam, Alexa 647-conjugated rabbit anti-mouse from Invitrogen; rabbit anti-β-tubulin from Cell Signaling Technology (Danvers, MA, USA); Alexa 488-conjugated goat anti-rabbit from Molecular Probes, Inc., (Eugene, OR, USA), mouse anti-O-GlcNAc (RL2) from Thermo Fisher Scientific; mouse anti-GAPDH from Santa Cruz Biotechnoloigy Inc (Sta. Cruz, CA, USA).

Fuentes-García G., Castañeda-Patlán M.C., Vercoutter-Edouart A.S., Lefebvre T, & Robles-Flores M. (2019). O-GlcNAcylation Is Involved in the Regulation of Stem Cell Markers Expression in Colon Cancer Cells. Frontiers in Endocrinology, 10, 289.

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