Flag m2 flag

Cell lysates were subjected to immunoblotting using the following antibodies: TY1 (clone MAb-054-050; Diagenode, Denville, NJ, USA), tubulin (clone B-5-1–2; Santa Cruz Biotechnology, Dallas, TX, USA), HA (clone 3F10; Roche, Penzberg, Germany), Akt1 (clone C-20; Santa Cruz Biotechnology), Phospho-Akt (Thr308) (clone D25E6; Cell Signaling Technology, Danvers, MA, USA), Phospho-Akt (Ser473) (clone D9E; Cell Signaling Technology), PP2A (clone 1D6; Millipore, Billerica, MA, USA), Phospho-PP2A alpha (clone E155; Abcam, Cambridge, UK), FLAG (M2 FLAG; Sigma-Aldrich, St. Louis, MO, USA), ERK1/2 (clone C-16/C-14; Santa Cruz Biotechnology), and I2PP2A (SET) (clone E-15; Santa Cruz Biotechnology). Immunoprecipitation was performed in an immunoprecipitation buffer (150 mM NaCl, 50 mM Tris pH 7.5, 1 mM EDTA, 1% Triton, 2 mM sodium orthovanadate, 2 mM PMSF, 50 mM sodium fluoride) as previously reported.^{14 (link)}