Spr 835

Manufactured by Millar

Sourced in Canada

The SPR 835 is a surface plasmon resonance (SPR) instrument. It is designed to detect and analyze molecular interactions in real-time. The device utilizes the principle of SPR to monitor changes in the refractive index near a metal surface, which can provide information about binding events between molecules.

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Lab products found in correlation

Vevo 770, by Fujifilm (3 mentions) Power laboratory system, by ADInstruments (2 mentions) Pvan software, by Millar (1 mentions) Vevo 2100 high resolution imaging system, by Fujifilm (1 mentions) Vevo 770 system, by Fujifilm (1 mentions) Isoflurane, by Baxter (1 mentions)

7 protocols using spr 835

Cardiac Function Assessment via Echocardiography

Cited 1 time

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Upon anesthetization, echocardiography was performed with a 30-MHz high-frequency scanhead (VisualSonics Vevo770, VisualSonics) as described previously.⁴³ (link) To monitor LV catheterization, a catheter manometer (Millar 1.4F, SPR 835, Millar Instruments) was inserted via the right carotid artery into the left ventricle. After stabilization, data were continuously recorded. Cardiac function parameters were calculated with the PVAN software (Millar Instruments, Houston, TX, USA) as described.⁴³ (link)

Li H., Dai B., Fan J., Chen C., Nie X., Yin Z., Zhao Y., Zhang X, & Wang D.W. (2019). The Different Roles of miRNA-92a-2-5p and let-7b-5p in Mitochondrial Translation in db/db Mice. Molecular Therapy. Nucleic Acids, 17, 424-435.

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Mouse Cardiac Function Assessment

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After being anaesthetized and placed on a platform, mice underwent echocardiography via the ultrasound machine Vevo2100 high‐resolution imaging system (VisualSonics). The instantaneous intraventricular pressure and volume were measured via inserting a 1.4‐F pressure‐volume catheter (SPR835, Millar Instruments) into the left ventricle of mice, as described previously.15

Fang Q., Liu T., Yu C., Yang X., Shao Y., Shi J., Ye X., Zheng X., Yan J., Xu D, & Zou X. (2020). LncRNA TUG1 alleviates cardiac hypertrophy by targeting miR‐34a/DKK1/Wnt‐β‐catenin signalling. Journal of Cellular and Molecular Medicine, 24(6), 3678-3691.

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Echocardiography and Pressure-Volume Analysis

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Echocardiography analysis was performed using a high-resolution imaging system with a 30-MHz high-frequency scanhead (VisualSonics Vevo770, VisualSonics, Toronto, Canada) as described previously.⁵⁴ (link) The pressure-volume catheter (Millar 1.4F, SPR835, Millar Instruments, Houston, TX) was inserted into the left ventricle to measure instantaneous intraventricular pressure and volume as described previously.¹¹ (link)

Nie X., Fan J., Li H., Yin Z., Zhao Y., Dai B., Dong N., Chen C, & Wang D.W. (2018). miR-217 Promotes Cardiac Hypertrophy and Dysfunction by Targeting PTEN. Molecular Therapy. Nucleic Acids, 12, 254-266.

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Invasive LV Hemodynamic Evaluation

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LV performance was further evaluated by invasive hemodynamic measurement using the Power Laboratory system (AD Instruments) with a 1.4-F micromanometric catheter (SPR 835, Millar Instruments) as we previously described.³⁵ (link)^,³⁸ Under stable anesthesia with 1.5% isoflurane, mice were placed in a supine position, a longitudinal incision (around 1 cm) was made in the middle of cervical area, the right common carotid artery was isolated, and the micromanometer was inserted into the artery and carefully advanced into the LV. LV end-systolic pressure (LVESP) and end-diastolic pressure (LVEDP) were acquired for evaluation of LV pressure, whereas maximal rate of pressure rising (Max dP/dt) and maximal rate of pressure fall (Min dP/dt) were derived for evaluation of LV systolic and diastolic function, respectively.

Li X., You J., Dai F., Wang S., Yang F.H., Wang X., Ding Z., Huang J., Chen L., Abudureyimu M., Tang H., Yang X., Xiang Y., Backx P.H., Ren J., Ge J., Zou Y, & Wu J. (2023). TAK1 Activation by NLRP3 Deficiency Confers Cardioprotection Against Pressure Overload-Induced Cardiomyocyte Pyroptosis and Hypertrophy. JACC: Basic to Translational Science, 8(12), 1555-1573.

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Evaluation of LV Hemodynamics in Mice

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Left ventricular hemodynamics was evaluated after 12 weeks by catheterization as previously described [23 (link)]. In brief, a micromanometer-tipped catheter (1.4 F, SPR 835; Millar Instruments, Houston, TX) was inserted through the right carotid artery into the aorta of an anaesthetized mouse and carefully introduced into the left ventricle (LV). The transducer was connected to a Power Laboratory system (AD Instruments, Castle Hill, New South Wales, Australia) and variables derived from catheterization included maximal ascending and descending rates of left ventricular pressure (±dP/dt), left ventricular end-systolic pressure (LVSP), left ventricular end-diastolic pressure (LVEDP), and heart rate.

He Q., Pu J., Yuan A., Yao T., Ying X., Zhao Y., Xu L., Tong H, & He B. (2014). Liver X receptor agonist treatment attenuates cardiac dysfunction in type 2 diabetic db/db mice. Cardiovascular Diabetology, 13, 149.

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Echocardiography and Pressure Measurement in Mice

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Mice were anaesthetized with 1.5% isoflurane (Baxter Healthcare Corporation). Echocardiographic images were obtained with a VisualSonics high-resolution Vevo 770 system (VisualSonics). Two-dimensional parasternal long axis views and short axis views were obtained at the papillary muscle level. Diastolic left ventricular posterior wall thickness (LVPWd) and systolic left ventricular posterior wall thickness (LVPWs) were measured and ejection fraction (EF) and fractional shortening (FS) were then calculated. All measurements were averaged for three consecutive cardiac cycles. To measure aortic and left ventricular (LV) pressure, a 1.4-F micromanometer conductance catheter (SPR-835; Millar Instruments) was introduced through the right common carotid artery into the ascending aorta and then advanced into the left ventricle as described previously [21 (link)].

Ma X., Fu Y., Xiao H., Song Y., Chen R., Shen J., An X., Shen Q., Li Z, & Zhang Y. (2015). Cardiac Fibrosis Alleviated by Exercise Training Is AMPK-Dependent. PLoS ONE, 10(6), e0129971.

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Transthoracic Echocardiography and Blood Pressure

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Transthoratic echocardiography was performed using 30 MHz high frequency scanhead (VisualSonics Vevo770, VisualSonics Inc. Toronto, Canada) [12, 13] . Blood pressure (BP) was measured by a micronanometer catheter (Millar 1.4F, SPR 835, Millar Instruments, Inc.) as previously described [12, 13, 19] (See Supplementary Material for more detailed information).

Li L., Zhou N., Kang L., Wang Q., Wu J., Wang X., Yang C., Zhang G., Chen Y., Jiang H., Chen R., Yang X., Sun A., Gong H., Ren J., Aikawa H., Komuro I., Ge J., Yang C., & Zou Y. (2020). Oxidized LDL but not angiotensin II induces the interaction between LOX-1 and AT1 receptors.

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